温度对肝片形吸虫在小土锅螺体内发育影响的观察

Study on Effect of Temperature on Fasciola Hepatica's Development in Galba Pervia

目的探讨温度对肝片形吸虫毛蚴感染小土蜗螺及毛蚴在其体内发育的影响,以期了解片形吸虫的生物学特征,为防控肝片形吸虫病提供科学依据。方法从云南省大理市下关泰兴市场收集病牛肝胆管及胆囊内的肝片形吸虫虫体并剥离出虫卵,孵化出毛蚴,在实验室分批进行人工感染小土蜗螺并观察不同温度下螺内虫体的发育状况。结果平均水温10℃时肝片形吸虫幼虫在小土蜗螺体内只能发育到胞蚴阶段,12℃时只能发育到母雷蚴阶段,18℃时只能发育到子雷蚴早期阶段,22℃时发育到尾蚴阶段。结论温度是影响肝片形吸虫幼虫在小土蜗螺体内发育的重要因素。

Objective To explore the influence of temperature on the miracidium of infection Galba pervia and miraeidium in its development, and to understand the biological characteristics of the fasciola hepatica. Methods hepatica eggs from the hepatic duet and gallbladder of cattle were collected from the shimonoseki taixing market of Dali city, miracidia were incubated and Galba pervia were infected with F. hepatica in the laborato- ry. Results Under average temperature 10℃, the shape of liver piece trematode larvae in small soil worm screw could only develop to cell larva stage; under 12℃ it developed to the mother redia stage; under 18℃ it only developed to early daughter redia, and in 22℃ it developed to the larvae stage. Conclusion The temperature is a key factor to infect the development of F. hepatica larvae.