活血降脂保肝汤对NAFLD大鼠肝组织MTP mRNA及蛋白表达的影响

Effect of Huoxue Jiangzhi Baogan Decoction on MTP mRNA and Protein Expressions in Hepatocytes of NAFLD Rats

目的:观察活血降脂保肝汤(HJBD)对非酒精性脂肪性肝病(NAFLD)大鼠肝组织微粒体甘油三脂转运蛋白(MTP)mRNA及蛋白表达的影响,探讨其防治NAFLD可能的作用机制。方法:取健康雄性SD大鼠60只随机分为6组,正常组,模型组,HJBD低、中、高剂量组(6.48,13.68,27.36 g·kg-1)及强肝片组(0.067 g·kg-1)。除正常组外,其余各组给予高脂饮食8周后,用低、中、高剂量HJBD及强肝片进行干预,连续ig 10周。采集空腹血分离血清检测各组大鼠血脂情况,取肝组织行苏木素-伊红(HE)观察大鼠肝组织病理变化,逆转录-聚合酶链式反应(RT-PCR)检测MTP mRNA及蛋白的表达情况。结果:免疫组化及RT-PCR检测结果显示,与正常组比较,模型组MTP表达水平降低,差异有统计学意义(P<0.01);与模型组比较,HJBD各剂量组及强肝片组MTP表达均升高,差异有统计学意义(P<0.01,P<0.05),以高剂量组升高最明显;血脂紊乱及肝脂肪变性也较模型组有不同程度改善。结论:HJBD对高脂饮食诱导的NAFLD大鼠有较好的治疗作用,推测MTP可能是HJBD抗NAFLD的有效作用靶点。

Objective： To observe the effects of Huoxue Jiangzhi Baogan decoction（ HJBD） on the mRNA and protein expression levels of microsomal triglyceride transfer protein（ MTP） in liver tissues of rats with non-alcoholic fatty liver disease（ NAFLD）,and explore its possible mechanisms of prevention and treatment for NAFLD. Method： Totally 60 healthy male SD rats were randomly divided into 6 groups： normal group,model group,HJBD high dose group,mid dose group,and low dose group（ 6. 84,13. 68,27. 36 g·kg- 1）,as well as Qianggan tablets group（ 0. 067 g·kg- 1）. All other groups except the normal group were given with high-fat diet for 8 weeks,and then the rats were either treated with low,middle or high doses of HJBD and Qianggan tablets through gastric gavage for 10 weeks. Fast serum samples were obtained for lipid profile. Liver histopathogy was performed on HE slides,and the expressions levels of MTP mRNA and protein in liver tissues were assayed by Real-time PCR. Result： Immunohistochemistry and RT-PCR results showed that,compared with the normal group,MTP expression was decreased in model group,with statistically significant difference（ P 〈 0. 01）.Compared with the model group,the expression levels of MTP were increased in various dose groups of HJBD and Qianggan tablets group,with statistically significant difference（ P 〈 0. 01,P〈 0. 05）,especially in high dose group. Blood lipids metabolic disorder and liver steatosis were also improved to varying degrees in the treatment groups as compared with the model group. Conclusion： HJBD has a good therapeutic effect for NAFLD rats induced by high-fat diet,and we speculate that MTP mRNA may be its effective targets.