七氟醚预处理对大鼠心肌细胞缺氧复氧损伤的保护作用及相关分子机制的探究

Exploration of protective effect of Sevoflurane preconditioning on hypoxia reoxygenation injury of myocardial cells in rats and related molecular mechanisms

目的：研究七氟醚预处理对大鼠心肌细胞缺氧复氧损伤的保护作用及相关分子机制。方法：培养出生后1~2dSD大鼠的心肌细胞,分为NC组（不做特殊处理）、HR组（缺氧复氧处理）、S组（七氟醚预处理后进行缺氧复氧）,测定心肌细胞损伤指标、线粒体途径凋亡指标以及氧化应激指标。结果：（1）心肌损伤指标：HR组心肌细胞的凋亡率以及细胞培养基中肌酸激酶（CK）、肌酸激酶同工酶（CKMB）以及谷草转氨酶（AST）、乳酸脱氢酶（LDH）的含量显著高于NC组（P〈0.05）,S组心肌细胞的凋亡率以及细胞培养基中CK、CK-MB、AST、LDH的含量显著低于HR组（P〈0.05）;（2）线粒体途径凋亡指标：HR组线粒体中Bcl-2、缝隙连接蛋白43（Cx43）、Omi/HtrA2的含量低于NC组（P〈0.05）,Bax的含量高于NC组（P〈0.05）;胞浆中细胞色素C、Omi/HtrA2含量高于NC组（P〈0.05）,Cx43含量低于NC组（P〈0.05）;S组线粒体中Bcl-2、Cx43、Omi/HtrA2的含量高于HR组（P〈0.05）,Bax的含量低于HR组（P〈0.05）,胞浆中细胞色素C、Omi/HtrA2含量低于HR组（P〈0.05）,Cx43含量高于HR组（P〈0.05）。（3）氧化应激指标：HR组细胞中活性氧（ROS）、丙二醛（MDA）的含量高于NC组（P〈0.05）,超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、VitC、VitE的含量低于NC组（P〈0.05）;S组细胞中ROS、MDA的含量低于HR组,SOD、GSH-Px、VitC、VitE的含量高于HR组（P〈0.05）。结论：七氟醚预处理对大鼠心肌细胞缺氧复氧损伤具有保护作用,其分子机制包括抑制线粒体途径凋亡、减轻氧化应激。

Objective：To explore the protective effect of Sevoflurane preconditioning on hypoxia reoxygenation injury of myocardial cells in rats and related molecular mechanisms.Methods：Cardiomyocytes of SD rats 1to 2dafter birth were cultured and divided into NC group（no special treatment）,HR group（hypoxia reoxygenation processing）and S group（hypoxia reoxygenation after sevoflurane preconditioning）.Myocardial cell injury indexes,mitochondrial pathway apoptosis indexes and oxidative stress indicators were detected.Results：（1）Myocardial injury indexes：myocardial cell apoptosis rate as well as CK,CK-MB,AST and LDH levels in cell culture medium of HR group were significantly higher than those of NC group,and myocardial cell apoptosis rate as well as CK,CK-MB,AST and LDH levels in cell culture medium of S group were significantly lower than those of HR group;（2）mitochondrial pathway apoptosis indexes：Bcl-2,Cx43 and Omi/HtrA2 contents in mitochondria of HR group were lower than those of NC group,and the content of Bax was higher than that of NC group;the contents of cytochrome C and Omi/HtrA2 in cytoplasm were higher than those of NC group,and the content of Cx43 was lower than that of NC group;the contents of Bcl-2,Cx43 and Omi/HtrA2 in mitochondria of S group were higher than those of HR group,and the content of Bax was lower than that of HR group;the contents of cytochrome C and Omi/HtrA2 in cytoplasm were lower than those of HR group,and the content of Cx43 was higher than that of HR group;（3）oxidative stress indexes：the contents of ROS and MDA in cells of HR group were higher than those of NC group,and the contents of SOD,GSH-Px,VitC and VitE were lower than those of NC group;the contents of ROS and MDA in cells of S group were lower than those of HR group,and the contents of SOD,GSH-Px,VitC and VitE were higher than those of HR group.Conclusions：Sevoflurane preconditioning has protective effect on hypoxia reoxygenation injury of myocardial cells in rats,and its molecular mechanisms include inhibiting mitochondrial pathway apoptosis and relieving oxidative stress.