糖尿病肾病患者血清Cys C、Hcy和尿mindin、NAG含量检测及对疾病早期诊断的价值

Detection of serum Cys C and Hcy as well as urine mindin and NAG contents in patients with diabetic nephropathy and value for early diagnosis of disease

目的:研究糖尿病肾病患者的血清胱抑素C(Cys C)、同型半胱氨酸(Hcy)和尿mindin、N-乙酰-β-D氨基葡萄糖苷酶(NAG)含量及对疾病早期诊断的价值。方法:选择糖尿病肾病患者和单纯糖尿病患者进行研究,采集血清并测定Cys C、Hcy、PGF-2α、丙二醛(MDA)、晚期蛋白氧化产物(AOPP)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、VitE的含量,采集尿液并测定mindin、NAG、MST1、JNK、Foxos、Caspase-3、Caspase-12的含量。结果:糖尿病肾病尿液中mindin、NAG、MST1、JNK、Foxos、Caspase-3、Caspase-12的含量以及血清中Cys C、Hcy、PGF-2α、MDA、AOPP的含量均显著高于单纯糖尿病患者(P<0.05),血清中SOD、GSH-Px、VitE的含量显著低于单纯糖尿病患者(P<0.05);CKD分期越高,尿液中mindin、NAG、MST1、JNK、Foxos、Caspase-3、Caspase-12的含量以及血清中Cys C、Hcy、PGF-2α、MDA、AOPP的含量越高,血清中SOD、GSH-Px、VitE的含量越低(P<0.05);尿液中mindin、NAG含量与MST1、JNK、Foxos、Caspase-3、Caspase-12含量呈正相关(P<0.05);血清中Cys C、Hcy含量与PGF-2α、MDA、AOPP的含量呈正相关(P<0.05),与SOD、GSHPx、VitE的含量呈负相关(P<0.05)。结论:糖尿病肾病患者血清Cys C、Hcy和尿mindin、NAG含量在CKD1期即开始升高,同时与细胞凋亡、氧化应激损伤密切相关,有助于疾病的早期诊断。

Objective：study the serum cystatin C（Cys C）and homocysteine（Hcy）as well as urine mindin and N-acetyl-β-D-glucosa minidase（NAG）contents in patients with diabetic nephropathy and the value for early diagnosis of disease.Methods：Patients with diabetic nephropathy and patients with diabetes alone were selected for study,serum was collected to detect Cys C,Hcy,PGF-2α,malondialdehyde（MDA）,advanced oxidation protein products（AOPP）,superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）and VitE contents,and urine was collected to detect mindin,NAG,MST1,JNK,Foxos,Caspase-3and Caspase-12 contents.Results：mindin,NAG,MST1,JNK,Foxos,Caspase-3and Caspase-12 contents in urine as well as Cys C,Hcy,PGF-2α,MDA and AOPP contents in serum of patients with diabetic nephropathy were significantly higher than those of patients with diabetes alone（P〈0.05）,and SOD,GSH-Px and VitE contents in serum were significantly lower than those of patients with diabetes alone（P〈0.05）;the higher the CKD stage,the higher the mindin,NAG,MST1,JNK,Foxos,Caspase-3and Caspase-12 contents in urine as well as Cys C,Hcy,PGF-2α,MDA and AOPP contents in serum,and the lower the SOD,GSH-Px and VitE contents in serum（P〈0.05）;mindin and NAG contents in urine were positively correlated with MST1,JNK,Foxos,Caspase-3and Caspase-12contents（P〈0.05）;Cys C and Hcy contents in serum were positively correlated with PGF-2α,MDA and AOPP contents（P〈0.05）,and negatively correlated with SOD,GSHPx and VitE contents（P〈0.05）.Conclusion：Cys C and Hcy in serum as well as mindin and NAG in urine of patients with diabetic nephropathy begin to increase from CKD1 stage,are closely related to cell apoptosis and oxidative stress injury,and help early diagnosis of the disease.