叶酸摄入量差异对大鼠同型半胱氨酸及腹主动脉的影响

Effects of different folic acid intakes on the homocysteine and abdominal aorta of rats

目的：通过给予大鼠不同量值的叶酸，观察叶酸对大鼠同型半胱氨酸（ Hcy）及腹主动脉的影响。方法选取30只成年雄性SD大鼠，按照随机数字表法分为无叶酸组、叶酸正常组及灌胃叶酸组。分别于第1周、第4周及第7周采取血清，测定血清叶酸及Hcy浓度。制作腹主动脉HE切片，于显微镜下观察内皮、内膜、中膜及外膜的变化。结果与无叶酸组相比，叶酸正常组和灌胃叶酸组第7周的血清叶酸浓度显著升高，差异有统计学意义（P＜0．05）；叶酸正常组和灌胃叶酸组的血清Hcy浓度显著降低，差异有统计学意义（P＜0．05）。显微镜观察显示，无叶酸组腹主动脉与叶酸正常组及灌胃叶酸组无明显差别，内皮细胞排列紧密，与内弹力膜紧贴在一起，未见溃疡、血栓、脱落及水肿等异常病变，内膜连续完整，中膜及外膜均无异常。结论短期缺乏叶酸及过量摄入叶酸不会使血清Hcy明显升高，长期缺乏叶酸可致血清Hcy明显升高。

Objective To observe the effects of different folic acid intake on homocysteine （ Hcy） and abdominal a-orta of rats.Methods A total of 30 adult male SD rats were randomly divided into 3 groups： a folic acid deficiency group, a normal folic acid group and an excessive folic acid group .The concentrations of Hcy and folic acid were meas-ured on Weeks 1, 4 and 7.The HE slice of the abdominal aorta were made and observed under a microscope for the changes of endothelial cells , internal membrance , medial membrance and outer membrance .Results On Week 7, re-markably increased levels of folic acid were measured in the normal folic acid group and the excessive folic acid group , compared with the folic acid deficiency group （P〈0.05）.The normal folic acid group and the excessive folic acid group produced markedly lower amounts of serum Hcy than the folic acid deficiency group （P〈0.05）.According to microscop-ic examination , the folic acid deficiency group showed close arrangement of endothelial cells , intact internal membrane and normal medial and outer membrances , which were similar with the normal folic acid group and the excessive folic acid group.Conclusion A short-term lack of folic acid can not lead to an obvious increase in serum Hcy .A long-term lack of folic acid can result in the rising of serum Hcy level .