琼脂糖膜固载辣根过氧化物酶催化还原过氧化物的研究

Study on Electrocatalysis of Peroxides by Horseradish Peroxidase Entrapped in Agarose Hydrogel Film

用琼脂糖(agarose)水凝胶将辣根过氧化物酶(HRP)固定在玻碳电极(GCE)表面,制备了HRP-Agarose膜修饰电极。在水-乙醇混合溶液中,包埋在琼脂糖水凝胶中的HRP可以与电极直接传递电子,并且能催化还原H2O2、过氧化丁酮、氢过氧化叔丁基、氢过氧化异丙基苯等过氧化物和NO。HRP-Agarose膜修饰电极具有较好的稳定性和重现性,可用于上述过氧化物和亚硝酸盐的定量检测。

Horseradish peroxidase was immobilized on the surface of a glassy carbon electrode（GCE）by agarose film.Horseradish peroxidase entrapped in the agarose film underwent a fast direct electron-transfer-reaction in water-ethanol solvent mixture,and showed electrocatalytic properties towards peroxides,such as hydrogen peroxide,cumene hydroper-oxide,t-butyl hydroperoxide,2-butanone peroxide and nitric oxide.The immobilized horseradish peroxidase had good stability and reproducibility,which could be applied to the determination of organic peroxides and nitrites.