基于分子量分布的生活污水荧光光谱研究

Fluorescence Spectroscopy Analysis of Domestic Wastewater Based on Molecular Weight Distribution

采用超滤及微滤膜法对生活污水进行分级,以荧光光谱为基本分析手段,结合荧光区域积分（FRI）法研究不同分子量区间组分的荧光特性。结果表明,不同分子量区间水样的同步荧光光谱图（SFS）中均含有3类荧光峰,分别为类蛋白质峰、类富里酸峰及类腐殖酸峰,其中类蛋白质峰荧光最强;类蛋白质、类腐殖酸及类富里酸的SFS区间积分强度大小顺序为类蛋白质区域〉类腐殖酸区域〉类富里酸区域,表明生活污水中蛋白质含量最高,腐殖酸次之,富里酸最少;各水样三维荧光光谱（EEM）图中主要存在4类荧光峰,分别为Peak T1、Peak T2、Peak A、Peak C,其中类蛋白质峰强度明显强于类腐殖酸;荧光区域积分（FRI）分析表明,类蛋白质占总标准体积60%~70%,进一步表明蛋白质为水体有机物的主要成分;膜孔径越小,类腐殖酸含量越高,腐殖化程度越高。

A domestic wastewater was classified by ultrafiltration and microfiltration membrane separation. The fluorescence characteristics of domestic wastewater in different molecular weight ranges were investigated by fluorescence spectrum and fluorescence regional integration（FRI）. The results showed that the synchronous fluorescence spectroscopy（SFS） of different molecular weight ranges all had three kinds of fluorescence peak ： protein-like fluorescence peak, fulvic-like peak and humic-like peak, the intensity of protein-like peak was relatively higher than others. The interval integral of SFS showed that protein-like regionhumic-like regionfulvic-like region. That was to say that the contents were protein-like humic-like fulvic-like. Three-dimensional fluorescence spectra（EEM） of all water samples had four fluorescence peaks which were Peak T1, Peak T2, Peak A and Peak C, and the protein-like peak was stronger than humic-like. The analysis of FRI showed that protein-like in the total standard volume was from 60% to 70%, also proved that the protein was the main component of the water organics, and the smaller membrane pores, the higher contents of the humic-like, the higher degree of humification.