摘要
对致病菌—尖孢镰刀菌（Fusrium oxysporum）具有较强拮抗作用的枯草芽孢杆菌（Bacillus subtilis）8-32产生的理化性质进行了初步研究。结果表明,该抗菌物质80℃处理30 min,拮抗活性仍保持在90.00%,100℃以上会完全丧失活性;在p H 7.0~11.0范围内抑菌活性较稳定,最适p H为8.0;高浓度（25mmol/L）EDTA使其拮抗活性降低62%;该抗菌物质对Cu2＋、Fe3＋敏感,对蛋白酶K不敏感。枯草芽孢杆菌8-32产生的抗菌物质具有一定的耐热、耐碱（p H 11.0）、对蛋白酶不敏感等特性。
The physicochemical properties of antibacterial substances secreted by Bacillus subtilis 8-32 which had strong antagonistic against Fusarium oxysporum were studied. The results showed that the antibacterial materials had good heat resistance, antagonist activity remained at 90.00% after 30 min treatment with 80 ℃ and completely lost above 100 ℃;The inhibitory activity of the antibacterial substances was stable in p H 7.0 ~11.0, and the best p H was 8.0; The antagonist activity reduced by 62% after added 25 mmol / L EDTA; The antibacterial substances were sensitive to Cu2 ＋、Fe3 ＋,but not sensitive to protease K. The experimental results showed that antibacterial substances extracted from B. subtilis 8-32 were heat-resistant, alkali resistant（p H 11.0）, and they were not sensitive to protease K.
出处
《湖北农业科学》
2016年第4期917-920,共4页
Hubei Agricultural Sciences
基金
河北省自然科学基金项目(C2015204031)
保定市科学技术研究与发展计划项目(13ZN023)
河北农业大学生命科学学院大学生创新项目
