化毒补虚方含药血清对人肺腺癌细胞增殖及凋亡的实验研究

Experimental Study on Function of Medicine-containing Serum of Huadu Buxu Formula on Cell Proliferation and Apoptosis of Human Pulmonary Adenocarcinoma

目的:观察不同浓度、不同时间化毒补虚方诱导肺腺癌细胞A549的抑制增殖作用及促进凋亡作用。方法:应用四氮唑蓝比色法(MTT法)检测化毒补虚方对人肺腺癌细胞增殖的影响,同时筛选出起有效抑制作用的浓度;并应用倒置显微镜观察细胞形态的变化及荧光显微镜、透射电子显微镜(TEM)观察细胞超微结构的变化。结果:MTT实验显示化毒补虚方抑制肺腺癌细胞增殖,其中50%浓度含药血清组对A549细胞抑制作用明显,在48 h达作用高峰;观察细胞形态及超微结构发现A549细胞形态发生明显改变,并可见凋亡小体。结论:化毒补虚方通过杀伤肺腺癌细胞、诱导细胞凋亡而发挥抗肿瘤作用。

Objective： to observe the function of Huayu Buxu Formula in inhibiting proliferation and facilitating apoptosis of A549 of pulmonary adenocarcinoma in different density at different time. Methods： MTT with Tertrazolium Blue was taken to detect the effect of Huayu Buxu Formula on the cell proliferation,and select the proper density with inhibiting function; an inverted microscope was used to observe cell changes in form,a fluorescence microscope and a transmission electron microscope to observe changes of cell ultrastructure. Results： MTT showed the formula could inhibit the cell proliferation,which was obvious in serum of 50% density and got to the peak at 48 hours; observing the ultrastructure found that A549 changed in form obviously and the apoptotic body could be seen.Conclusion： Huadu Buxu Formula plays a anti-tumor role by killing pulmonary adenocarcinoma cells and inducing apoptosis.