慢性运动性疲劳大鼠海马多种单胺类递质水平变化规律的研究

On different monoamine transmitter changes of the hippocampi in rats with chronic exercise-induced central fatigue

目的探讨慢性运动性疲劳状态下,大鼠海马组织各种单胺类递质的变化特点。方法建立慢性运动性中枢疲劳大鼠模型,造模时间为4周;对照组不采取任何干预措施,与模型组相同条件正常喂养,时间四周。所有实验动物取材部位为大鼠双侧海马,检测方法为高效液相色谱电化学检测法,研究指标选取5-羟色胺(5-HT)、去甲肾上腺素(NE)、肾上腺素(E)和多巴胺(DA)的含量进行定量分析。结果与对照组相比,模型大鼠在慢性运动性中枢疲劳状态下,其海马组织中5-羟色胺含量明显升高(P<0.01),同时多巴胺和肾上腺素的含量明显下降,二者均在统计学有显著性差异(P<0.01,P<0.05);而同样属于单胺类神经递质的去甲肾上腺素的水平则明显升高(P<0.01)。结论在中枢性疲劳发生时,大鼠海马内四种重要的单胺类神经递质的水平均发生了改变,与额叶皮质研究发现存在不同,这说明大鼠慢性运动性疲劳状态下中枢神经递质确实发生着明显的改变,同时这种变化在不同脑组织中存在着明显差异。

Objective The paper aims to study the changes of different monoamine transmitters in the hippocampus tissues of rats under chronic exercise- induced fatigue. Methods The Rats＇ Chronic Exercise- induced Central Fatigue Model was set within four weeks. For the control group,there was no intervention concerned and they were raised normally under the same condition as the rats with central fatigue for four weeks. For all the laboratory animals,the bilateral hippocampus of rats was taken as investigating material. The electrochemical detection of the high performance liquid chromatography was adopted to carry out the quantitative analyses of the content of 5- HT,epinephrine,norepinephrine and dopamine. Results Compared with the control group,it is easily found that there is a significant increase of 5- HT in the hippocampi of rats with exercise- induced central fatigue（ P〈0. 01）. At the same time,a statistically significant decrease of dopamine and epinephrine was found（ P〈0. 01,P〈0. 05）. However,there is also a significant increase of norepinephrine,a monoamine neurotransmitter. Conclusion With the rats＇ central fatigue,four important monoamine neurotransmitters have significant changes simultaneously,which are 5- HT,epinephrine,norepinephrine and dopamine respectively. The result is different from that of the study of the frontal cortex. And it explains that there exist significant changes of central neurotransmitters of the rats under chronic exercise- induced fatigue and the changes are significantly different in different brain tissues.