摘要
本研究以骨骼肌表型差异明显的两个绵羊品种特克塞尔羊和乌珠穆沁羊为试验对象,通过对两个与生长发育相关的基因卵泡抑素(follistatin,FST)与纤维蛋白聚糖基因(fibromodulin,FMOD)在不同日龄、不同骨骼肌部位表达的比较分析,探讨绵羊骨骼肌早期发育规律;同时对绵羊胎儿的85、100、120和135日龄4个阶段,在背最长肌、半腱肌、股四头肌、半膜肌和股二头肌5个部位肌肉FST和FMOD基因的表达量进行了比较研究。实时荧光定量PCR结果表明,随着胎儿日龄的增加,FST基因在半腱肌、股四头肌中表达量有增加的趋势,同时在特克塞尔绵羊中的表达量高于乌珠穆沁羊。在100和120日龄半腱肌、120日龄半膜肌、120日龄股二头肌中FST基因表达量在两个品种间差异显著(P<0.05),在100和135日龄半膜肌、85日龄背最长肌、100日龄股四头肌、100日龄股二头肌中FST基因表达量在两个品种间差异极显著(P<0.01)。FMOD基因在股四头肌中表达量较高,而在其他4种肌肉组织中相对表达量较低,且在乌珠穆沁羊中的表达量高于特克塞尔羊中的表达量。FST和FMOD基因在绵羊早期骨骼肌发育中起着重要作用。
In order to investigate skeletal muscle development mechanism,the expression of follistatin(FST)and fibromodulin(FMOD)gene were comparatively analyzed in fetus different skeletal muscles and different stages between Ujumqin sheep and Texel sheep during early stage.Two gene expression were analyzed including longissimusdorsi,semimembranosus,semitendinosus quadricepsfemoris and biceps femoris muscle were compared by variance analysis methods of four stages(85,100,120 and 135d).The results of quantitative Real-time PCR showed that the expression of FSTgene showed a tendency to increase with the addition of stage,and the expression of FSTgene in Texel sheep was higher than that in Ujumqin sheep in semitendinosus(100,120d),semimembranosus(120d),and biceps femoris(120d)(P〈0.05).The expression of FSTgene in Texel sheep was extremly higher than that of Ujumqin sheep in semitendinosus(100,135d),longissimusdorsi(85d),quadricepsfemoris(100d)and biceps femoris(100d)(P〈0.01).As a whole,the expression of FMODgene was the highest in quadriceps femoris,and was lower in other four muscles,it's expression in Ujumqin sheep was higher than that in Texel sheep.It was inferred that FSTand FMODgenes might be related to muscle development during early stage.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第3期720-729,共10页
China Animal Husbandry & Veterinary Medicine
基金
国家科技支撑计划项目:优质肉羊新品种(系)选育与关键技术研究及示范(2011BAD28B05-2)