摘要
目的:筛选禹州漏芦保护肝损伤的有效部位,并分析其对四氯化碳(CCl4)引起的小鼠急性肝损伤的保护机制。方法:将110只昆明种小鼠随机分为11组,包括正常组,模型组,阳性组,水高剂量、低剂量组,正丁醇高剂量、低剂量组,乙酸乙醇高剂量、低剂量组,石油醚高剂量、低剂量组。分别每天给予不同剂量的药物:阳性药组(25 mg·kg^(-1)),石油醚高剂量、低剂量组(138.65、69.33 mg·kg^(-1)),乙酸乙醇高剂量、低剂量组(40、20 mg·kg^(-1)),正丁醇高剂量、低剂量组(119.95、59.98 mg·kg^(-1))及机制。方法:将110只昆明种小鼠随机分为11组,包括正常组,模型组,阳性组,(175.79、87.40 mg·kg^(-1)),正常组和模型组给予等体积的羧甲基纤维素钠溶液。7 d后,除正常组外各组小鼠腹腔注射0.2%CCl4花生油溶液制备急性肝损伤模型,16 h后取血检测血清中谷草转氨酶(aspertate aminotransferase,AST)、谷丙转氨酶(alanine transaminase,ALT)活性;测定肝匀浆中丙二醛(malondialdehyde,MDA)含量和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)的活性及观察肝脏病理学变化,观察各组肝损伤。结果:模型组肝脏系数为5.3±2.3,ALT为(111.61±22.21)U·L^(-1),AST为(244.08±55.09)U·L^(-1),MDA为(15.53±10.01)nmol·mg^(-1),SOD为(56.03±26.66)U·mg^(-1),GSH-Px为(102.23±53.71)μmol·mg^(-1)。正丁醇高剂量、低剂量组肝脏系数分别为4.6±2.8、4.7±3.3,ALT分别为(66.95±28.01)U·L^(-1)、(81.83±29.54)U·L^(-1),AST分别为(135.29±89.05)U·L^(-1)、(163.39±77.83)U·L^(-1),MDA分别为(7.90±1.90)nmol·mg^(-1)、(9.25±3.31)nmol·mg^(-1),SOD分别为(94.30±29.22)U·mg^(-1)、(88.03±27.84)U·mg^(-1),GSH-Px分别为(208.22±71.63)μmol·mg^(-1)、(176.89±72.48)μmol·mg^(-1)。水高剂量、低剂量组肝脏系数分别为(4.6±3.4)、(4.7±4.9),ALT分别为(74.50±29.28)U·L^(-1)、(83.17±30.60)U·L^(-1),AST分别为(150.23±81.31)U·L^(-1)、(175.34±80.87)U·L^(-1),MDA分别为(8.49±4.45)nmol·mg^(-1)、(9.00±4.93)nmol·mg^(-1),SOD分别为(80.86±23.96)U·mg^(-1)、(78.03±20.20)U·mg^(-1),GSH-Px分别为(205.07±62.91)μmol·mg^(-1)、(162.21±46.46)μmol·mg^(-1)。正丁醇高剂量、低剂量组,水高剂量、低剂量组以上指标与模型组比较,差异均有统计学意义(P<0.05)。光镜下可见,正丁醇高剂量、低剂量组,水高剂量、低剂量组均可明显缓解小鼠急性肝损伤。结论:禹州漏芦保护肝脏的药效学活性部位为正丁醇部位和水部位,其保护肝脏的作用机制与其抗脂质过氧化有关。
Objective: To study and screen the effective part of Echinopsis Radix and mechanism of action in carbon tetrachloride( CCl4) induced acute liver injury in mice. Methods: 110 mice were randomly divided into 11 groups as follows: the drug groups mice were treated with positive medicine( 25 mg·kg^-1),Petroleum ether extraction( 138. 65,69. 33mg·kg^-1),acetic ether extraction( 40,20 mg·kg^-1),n-butyl alcohol extraction( 119. 95,59. 98mg·kg^-1) and aqueous extraction( 175. 79,87. 40 mg·kg^-1) for 7 day. At the same time,the normal control group and the model control group took equivalent amount of CMC-Na solution by the same route. And mice in the positive control group were treated with 25 mg /( kg ·d)- 1biphenyl dimethyl dicarboxylate. After the last administration,all groups except the normal control were injected with 0. 2% CCl4 solution to induce acute hepatic injury. Thereafter,the contents of ALT and AST in serum; the activity of MDA,SOD and GSH in the liver were measured and the hepatic histological changes were observed by optical microscope. Results: The liver coefficient in the model group was( 5. 3 ±2. 3),ALT was( 111. 61 ± 22. 21) U·L^-1,AST was( 244. 08 ± 55. 09) U·L^-1,MDA was( 15. 53 ± 10. 01) nmol·mg^-1,SOD was( 56. 03 ± 26. 66) U·mg^-1,GSH-Px was( 102. 23 ± 53. 71) μmol·mg^-1. The liver coefficient in the n-butyl alcohol high dose and low dose group respectively was( 4. 6 ± 2. 8) and( 4. 7 ± 3. 3),ALT was( 66. 95 ± 28. 01) U·L^-1and( 81. 83 ±29. 54) U·L^-1respectively,AST was( 135. 29 ± 89. 05) U·L^-1and( 163. 39 ± 77. 83) U·L^-1respectively,MDA respectively was( 7. 90 ± 1. 90) nmol·mg^-1and( 9. 25 ± 3. 31) nmol·mg^-1and SOD was( 94. 30 ± 29. 22) U·mg^-1and( 88. 03 ± 27. 84)U·mg^-1,GSH PX was( 208. 22 ± 71. 63) μmol·mg^-1and( 176. 89 ± 72. 48) μmol·mg^-1. The liver coefficient in the aqueous extraction high dose and low dose group respectively was( 4. 6 ± 3. 4) and( 4. 7 ± 4. 9),ALT was( 74. 50 ± 29. 28) U·L^-1and( 83. 17 ± 30. 60) U·L^-1respectively,AST was( 150. 23 ± 81. 31) U·L^-1and(( 175. 34 ± 80. 87) U·L^-1respectively,MDA respectively was( 8. 49 ± 4. 45) nmol·mg^-1and( 9. 00 ± 4. 93) nmol · mg^-1and SOD was( 80. 86 ± 23. 96) U · mg^-1and( 78. 03 ± 20. 20) U·mg^-1,GSH-PX was( 205. 07 ± 62. 91) μmol·mg^-1and( 162. 21 ± 46. 46) μmol·mg^-1. The above indexes in the n-butyl alcohol high dose and low dose group,aqueous extraction high dose and low dose group compared with the model group showed that the differences were statistically significant( P〈0. 05). Under the light microscope,it was found that n-butyl alcohol high dose and low dose group,aqueous extraction high dose and low dose group could obviously alleviate the acute liver injury in mice. Conclusion: The results show that the pharmacody namicactive extracts of Echinopsis Radix are the n-butanol and water fractions. The mechanism of liver protection action is apply to it anti-lipid peroxidation.
出处
《中医学报》
CAS
2016年第2期232-237,共6页
Acta Chinese Medicine
基金
河南省科技厅重点科技攻关项目(122102310185)
关键词
禹州漏芦
活性部位
肝功能
小鼠
Echinopsis Radix
active site
liver function
mice