补充足量锌对脊髓缺血再灌注损伤大鼠神经功能的作用

Effect of Supplement of Full Dose of Zinc on Neurological Function of Rats with Spinal Cord Ischemia- reperfusion Injury

目的探讨补充足量锌对大鼠脊髓缺血再灌注损伤的神经保护作用。方法雄性SD大鼠40只,建立脊髓缺血再灌注损伤模型,实验分假手术组、模型组、高锌饮食组和低锌饮食组。采用BBB评分及足迹分析对各组大鼠术后后肢运动情况进行评价,检测各组Neu N阳性神经元数量。结果高锌饮食组BBB评分在实验的各时间点都高于其他3组,差异有统计学意义(P<0.01);足迹分析显示高锌饮食组可明显改善大鼠步长(P<0.05),步宽无明显变化;术后各时间点,高锌饮食组大鼠Neu N阳性神经元数量明显高于模型组和低锌饮食组。结论补充足量锌可促进脊髓缺血再灌注损伤大鼠后肢运动功能的恢复,对神经元损伤起保护作用。

Objective To investigate the effect of the supplement of full doze of zinc on the neurological function of rats with spi- nal cord ischemia - reperfusion injury. Methods The models of spinal cord I/R injury were established with 40 male SD rats. They were divided into four groups： sham operation group, model group, zinc -high dietary group （ZH） and zinc -deficient dietary group （ZD）. The hindlimb motor function of the rats in the four groups was evaluated with BBB score and footprint analysis, and the number of NeuN - positive cells was also measured. Results The rats in the ZH group had higher BBB scores at any time during the experi- ment than those in the other three groups, with statistically significant difference （P 〈 0. 01 ）. The footprint analysis showed that the stride length of the rats in the ZH group was significantly improved （ P 〉 0. 05 ） , while the stride width was not. At each time point af- ter operation, the number of NeuN - positive cells of the rats in the ZH group was significantly higher than that in the model group and the ZD group. Conclusion The supplement of full doze of zinc can help the recovery of the hindlimb motor function of the rats with spinal cord ischemic reperfusion injury, and protect them from nerve injury.