HPLC法同时测定不同采收期江枳壳中辛弗林和N-甲基酪胺的含量

SIMULTANEOUS DETERMINATION OF SYNEPHRINE AND N-METHYLTYRAMINE IN JIANGXI AURANTII FRUCTUS AT DIFFERENT HARVEST TIME BY HPLC

目的采用HPLC法同时测定不同采收期江枳壳中辛弗林和N-甲基酪胺的含量,考察不同采收期江枳壳中上述两种生物碱含量的变化。方法采用高效液相色谱法。结果辛弗林与N-甲基酪胺分别在1.09～5.45μg（R2=0.9997）,0.18～0.90μg（R2=0.9999）范围内呈良好的线性关系;其平均回收率（n=6）分别为102.30%和98.07%,RSD分别为2.12%和2.20%;江枳壳样品中辛弗林和N-甲基酪胺的含量分别在0.07%～0.43%和0%～0.06%的范围内。结论辛弗林含量随采收期的推迟而降低,N-甲基酪胺在当年7月份以后检测不到含量。该方法简便、准确、灵敏度高,可用于辛弗林和N-甲基酪胺的含量测定。

Objective：To determine the contents of synephrine and N-methyltyramine in Jiangxi aurantii fructus at different harvest time by HPLC, and to investigate the changes of the two components. Methods： HPLC was applied in the experiment. Results： The method had good linear relationship within the range of 1.09～5.45 μg（R2 =0.9997）for synephrine and 0.18～0.90 μg（R2 = 0.9999）for N-methyltyramine. The average recoveries（ n = 6） were 102.30% with RSD of 2.12% for synephrine and 98.07% with RSD of 2.20% for N-methyltyramine respectively. The contents in Jiangxi aurantii fructus were 0.07%～0.43% for synephrine and 0～0.06% for N-methyltyramine respectively. Conclusion： The content of synephrine were low at late harvest time, N-methyltyramine wasn＇t detected since July. The method is convenient, accurate and highly sensitive, and can be applied to the determination of synephrine and N-methyltyramine in aurantii fructus.