检测人乳腺癌MCF-7细胞在不同HER-2水平培养上清中miRNAs的表达谱

Expression profile of miRNAs in supernatant of cultured human breast cancer MCF-7 cells at different HER-2 levels

目的检测不同人类表皮生长因子受体-2(human epidermal growth factor receptor-2,HER-2)表达水平在人乳腺癌细胞培养上清中微小RNAs(miRNAs)的表达,初步筛选与HER-2表达相关的miRNAs表达谱。方法培养人乳腺癌细胞MCF-7和稳定转染HER-2的MCF-7(MCF-7/HER-2),分别收集2株细胞的培养上清,利用miRNAs芯片技术检测其miRNAs的表达,并与MCF-7和MCF-7/HER-2细胞中miRNAs表达谱进行对比。结果细胞培养上清中筛选138种与HER-2相关的miRNAs,较正常MCF-7细胞上调86个,下调52个。与细胞中筛选的miRNAs对比,同时上调与下调各有7种;在细胞培养上清液中上调而在细胞中下调的有1种;在细胞培养上清液中下调而在细胞中上调的有7种。结论不同HER-2水平的人乳腺癌细胞外泌miRNAs存在差异性表达。

Purpose To detect the expression of miRNAs in the supernatant of cultured human breast cancer cells with different human epidermalgrowth factor receptor-2 （HER-2） expression levels, and to preliminarily screen HER-2 expression related miRNAs profile. Methods Two human breast cancer cell strains, MCF-7 and MCF-7/HER-2 which is stably transfected by HER-2, were cultured. The expression profile of miRNAs in the supernatant and cultured cells were detected by miRNAs microarray analysis and compared. Results In the supematant of cultured eel]s, 138 kinds of miRNAs relevant to HER-2 were selected. Compare with HER-2-negative MCF-7 cells, 86 were up-regulated and 52 were down-regulated. Among those differentially expressed miRNAs, 7 of them were up-reg- ulated and 7 were down-regulated in the supernatant and the cells. Meanwhile, 1 up-regulated miRNA in the supernatant was down- regulated in the cells, and 7 down-regulated miRNAs in the supernatant were up-regulated in cells. Conclusion Differential excretion of miRNAs does exist in human breast cancer cells with different HER-2 levels.