小剂量辐射致小鼠血液中microRNA表达改变

microRNA expression changes induced by low-dose irradiation in mice

目的探讨^(60)Coγ射线小剂量辐射损伤对小鼠血液microRNA的影响及意义。方法 SPF级C57BL/6J小鼠接受0.5 Gy全身照射后,分别于照射后6、24 h进行外周血白细胞计数。同时应用Agilent microRNA生物芯片筛选小鼠血液中差异表达microRNA。结果 microRNA芯片筛选出辐射后6 h小鼠中差异表达miRNA共11个,其中7个上调,4个下调。照射24 h小鼠中差异表达miRNA共32个,其中13个上调,19个下调。且与未照射组比较表达差异有统计学意义(P<0.05)。0.5 Gy照射后,6 h和24 h外周血白细胞总数与对照组相比差异无统计学意义(P>0.05)。miRNA表达变化优于血液中白细胞的变化。结论 microRNA在血浆中差异表达与辐射损伤有关,其有望成为辐射损伤新的血液标志物。

Objective To explore the impact and significance of low- dose ^60Coγ- ray irradiation on peripheral blood microRNA（miRNA） expression profiles in mice. Methods SPF C57BL/6J mice were exposed to a single dose of 0.5 Gy whole - body irradiation, and the total number of peripheral WBC was measured at 6h and 24 h after the exposure. The differential expression of miRNA in peripheral blood was simultaneously analyzed by Agilent miRNA microarrays. Results Six hours after the irradiation, miRNA microarray revealed that 11 miRNAs were differentially expressed, in which 7 miRNAs were up- regulated and 4 miRNAs downregulated. 32 miRNAs were differentially expressed at 24h after the irradiation, in which 13 miRNAs were up - regulated and 19 miRNAs down - regulated, microRNA expression had significant differences between the irradiation group and the non- irradiation group（ P 〈 0.05）. No statistically significant difference was found in the total number of peripheral WBC at 6h and 24h after 0.5 Gy wholebody irradiation between the two groups（P 〉 0.05）. The changes of microR- NA expression were better than those of peripheral W BC. Conclusions Differential expression of miRNA in peripheral blood is associated with irradiation damage, and it may be considered as a new biomarker for irradiation damage.