摘要
目的研究红外线对体外培养成纤维细胞(HSF)中c-Jun和Ⅰ、Ⅲ型胶原表达的影响,为红外线引起光老化损伤的分子机制提供理论依据。方法提取原代皮肤成纤维细胞培养,成纤维细胞被分为对照组(无红外线照射)和实验组(红外线照射);用MTT检测各组细胞活性;用实时定量PCR和免疫细胞化学(ICC)方法检测各组c-Jun和Ⅰ、Ⅲ型胶原m RNA和蛋白的表达。结果MTT检测显示与对照组比较,实验组各组中红外线照射对HSF的增殖有不同程度的抑制作用;红外线照射下调Ⅰ型胶原m RNA和蛋白的表达,随着照射剂量的增加,Ⅰ型胶原的m RNA和蛋白表达下降越明显(P<0.01);红外线照射成纤维细胞12 h后Ⅲ型胶原m RNA和蛋白的表达都呈照射剂量依赖性下调(P<0.05,P<0.01),24 h后Ⅲ型胶原m RNA和蛋白的表达都呈照射剂量依赖性上调(P<0.05,P<0.01);红外线照射上调了c-Jun m RNA和蛋白的表达水平,随着照射剂量的增加,呈照射剂量依赖性(P<0.05,P<0.01)。结论红外线照射人皮肤成纤维细胞后上调c-Jun表达,抑制Ⅰ型胶原表达,干扰Ⅲ型胶原表达,这可能是其引发和促进皮肤光老化的发生机制之一。
Objective To observe the effect of solar infrared ray(IR) radiation on the expressions of c-Jun and collagens I and III in cultured human skin fibroblasts(HSFs) and explore the molecular mechanism by which IR radiation causes aging of the skin. Methods Primarily cultured HSFs exposed to IR radiation were examined for changes of the cell viability with MTT assay. The m RNA and protein expressions of c- Jun and collagens I and III was detected with real- time quantitative PCR and immunocytochemistry. Results MTT assay showed that IR irradiation caused inhibition of cell proliferation compared with the control cells. The m RNA and protein expression of collagen I was decreased significantly by IR irradiation with the increase of the irradiation dose(P〈0.01). HSFs irradiated by IR for 12 h showed a dose-dependent reduction of the expression of collagen type III m RNA and protein(P〈0.05, P〈0.01), but the expression increased dose-dependently in response to IR exposure for 24 h(P〈0.05 or 0.01). IR irradiation enhanced the m RNA and protein expression of c-Jun in a dose-dependence manner(P〈0.05 or0.01). Conclusion IR irradiation can increase the expression of c-Jun, inhibit the expression of collagen I, and cause disturbance in collagen III expression in human skin fibroblasts, which may be one of the mechanism of IR radiation to initiate and promote skin photoaging.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2016年第2期163-169,共7页
Journal of Southern Medical University
基金
国家自然科学基金(30600764)
西安交通大学第二医院院基金重点项目(YJ(ZD)201307)~~