大鼠肾脏细胞17β-HSD1的表达及参与性激素合成的能力

Expression of 17 beta-hydroxysteroid dehydrogenase type 1 in the kidney of rats: the capacity of the kidney for synthesizing sex hormones

目的通过研究合成性激素的关键酶17β-HSD1在肾脏中的表达,探讨肾脏是否具备合成性激素的作用。方法基于无促卵泡生成素(FSH)、黄体生成素(LH)培养基和有FSH、LH培养基两种条件下,Western blotting、放射免疫分析法分别检测培养24、48 h后肾脏细胞中17β-HSD1的表达和性激素的分泌情况。结果培养24 h后,大鼠肾脏细胞能够表达少量的17β-HSD1蛋白(0.1843±0.076),同时能够分泌少量的雌二醇、孕酮和睾酮(分别为3.30±3.78 nmol/L,62.60±12.33 pmol/L和22.12±3.36 nmol/L),而在FSH和LH的共同刺激下,大鼠肾脏细胞17β-HSD1蛋白的表达量明显升高(1.6651±0.044,P<0.01),同时分泌雌二醇、孕酮和睾酮的量也显著增加(分别为8.50±2.64 nmol/L,117.80±9.79 pmol/L和45.04±4.39 nmol/L,均P<0.05),培养24h和48 h上述指标均无明显差异(P>0.05)。结论大鼠肾脏细胞中有17β-HSD1的表达,并且在FSH和LH的共刺激下能够稳定分泌性激素,提示肾脏组织具备合成性激素的能力,丰富了肾脏的内分泌功能。

Objective To investigate the expression of 17 beta-hydroxysteroid dehydrogenase type 1（17β-HSD1） in the kidney of rats and explore the capacity of the kidney for synthesizing sex hormones. Methods The expressions of 17- HSD1 and sex hormones were detected by Western blotting and radioimmunoassay in rat renal cells in primary cultured for 24 and 48 h in the presence or absence of follicle-stimulating hormone（FSH） and luteinizing hormone（LH）. Results After cell culture for 24 h, the primary rat renal cells expressed a low level of 17β- HSD1（0.1843 ± 0.076）, which increased to 1.6651 ± 0.044（P〈0.01） in response to co- stimulation by FSH and LH. Low levels of estradiol, progesterone and testosterone were also detected in rat renal cells（3.30±3.78, 62.60±12.33, and 22.12±3.36, respectively）, and co-stimulation of FSH and LH significantly increased their levels to 8.50 ± 2.64, 117.80 ± 9.79, and 45.04 ± 4.39, respectively（P〈0.05）. The levels of these hormones showed no significant differences between cells cultured for 24 h and 48 h（P〈0.05）. Conclusion The rat renal cells express 17β-HSD1 and are capable of stably secreting sex hormones in response to co-stimulation with FSH and LH, suggesting the capacity of the rat kidneys for synthesizing sex hormones. These findings enrich the understanding of the endocrine function of the kidney.