超滤技术结合大孔吸附树脂纯化低聚果糖

Purification of fructo-oligosaccharide by membrane separation process combined with macroporpus resin

菊粉酶解制备低聚果糖（FOS）过程中,酶解液中通常含有一定量的单糖、蔗糖和未酶解完全的菊粉;为了制备高纯度低聚果糖,酶解液先后经超滤（除去未完全酶解的菊粉）和大孔吸附树脂纯化（分离FOS、单糖和蔗糖）处理。因此,探讨超滤技术最佳工艺参数,比较4种树脂（CSR-1Na、CSR-2Na、CSR-3Na、CSR-1Ca）对FOS的纯化效果,选择最佳树脂并对其纯化工艺参数进行优化。结果表明,超滤技术的最佳操作工艺参数为：操作压差1.0 MPa,循环流量2L/min,pH6.0;该条件下,超滤的渗透通量为10.8L/（m2·h）,FOS透过率为93.7%,纯度为63.84%。CSR-1Na型树脂对FOS的纯化效果较好;大孔树脂最佳纯化工艺参数为：操作温度60℃,体积流速1.5mL/min,操作pH 6.0~6.5;该条件下FOS（纯度大于95%）的回收率为83.26%。

A certain amount of glucosefructose,sucrose and inulin are still exist in the solution of fructo-oligosaccharide which is obtained by hydrolysis of inulin.Fos solution was purified by the membrane separation process and macroporpus resin.The purification effect for Fructo-oligosaccharide of four kinds of resins（CSR-1Na,CSR-2Na,CSR-3Na,CSR-1Ca）was compared,the optimum was CSR-1Na.The effects of filtration pressure,circulation flow rate,pH on the transmittance of sugars and the permeation flux were studied.The optimum conditions for ultrafiltration were filtration pressure of10 bar,pH of 6.0,circulation flow rate of 2L/min,twice and the transmissivity ratio of FOS was 93.7%,the permeation flux was10.8L/（m2#h）,the purity of glucosefructose was 63.84%.The optimum conditions for purification of macroporpus resin were temperature of 60 ℃,volumetric flow rate of 1.5mL/min,pH of 6.0~6.5and the type of 95 fructooligosaccharides recovery rate was 83.26%.