白藜芦醇对LPS诱导HUVEC细胞线粒体跨膜电位影响研究

Effects of resveratrol on mitochondrial membrane potential of HUVEC cells induced by LPS

目的:探讨白藜芦醇对LPS刺激HUVEC细胞活性及线粒体跨膜电位影响。方法:体外培养HUVEC,随机分为LPS组、阴性组、白藜芦醇160、80、40、20μg·l-1剂量组。其中,LPS组与白藜芦醇各组分别以1μg·ml-1 LPS刺激细胞24h后,采用CCK-8法检测细胞活力,荧光显微镜下观察罗丹明染色后细胞线粒体跨膜电位。结果:白藜芦醇各组均能提高LPS诱导的HUVEC细胞存活率,提高细胞线粒体跨膜电位,与模型组比较有统计学差异(P<0.05)。结论:白藜芦醇能提高LPS刺激HUVEC细胞活性,提高细胞线粒体跨膜电位。

Objective：To investigate the effects of resveratrol on the mitochondrial membrane potential of the HUVEC cells induced by LPS.Methods：HUVEC cells were cultured in vitro and divided into LPS group,normal group,160,80,40,20μg·L-1resveratrol group.LPS group and resveratrol groups were stimulated by 1μg·m l^-1 LPS for 24 h,cell viability was observed by CCK-8;mitochondrial membrane potential were tested by the fluorescence microscope.Results：Compared with model group,resveratrol at the concentration of 160,80,40,20μg·L-1could increase the cell viability and increase the mitochondrial membrane potential significantly（P〈0.05）.Conclusion：Resveratrol could against the apoptosis of HUVEC cell induced by LPS,and the mechanism may be associated with the increasion of the mitochondrial membrane potential.