来氟米特对大鼠肾小球系膜细胞增殖的影响

Effects of Leflunomide on the Proliferation of the Rat Mesangial Cells

目的:探讨来氟米特(A771726)对大鼠肾小球系膜细胞(RMC)增殖的影响。方法:体外培养RMC,随机分为正常对照组、A771726(25μg/ml)组、A771726(50μg/ml)组、A771726(75μg/ml)组,干预培养48h后,采用CCK-8法检测各组RMC的增殖情况,流式细胞术检测各组RMC细胞周期的情况。结果:与正常对照组相比,A771726(25μg/ml)组、A771726(50μg/ml)组、A771726(75μg/ml)组RMC的增殖均被显著抑制,G1期细胞均显著增加,S期细胞均显著减少,细胞周期被阻滞于G1期(均P<0.05),并呈明显浓度依赖性。结论:来氟米特(A771726)可显著抑制RMC细胞的增殖,细胞周期被阻滞于G1期,且随着浓度的升高,其抑制作用越显著,呈明显浓度依赖性。

Objective：To investigate the effects of leflunomide（A771726）on the proliferation of the rat mesangial cells.Methods：The cultured rat glomerular mesangial cells were divided into the normal control group,A771726（25μg/ml）group,A771726（50μg/ml）group,and the A771726（75μg/ml）group.Afterr 48 hours treatment,cell proliferation was measured by cell counting kit-8（CCK-8）assay,cell cycle was measured by flow cytometry.Results：Compared with the normal control,A771726 could significantly inhibit the proliferation of rat glomerular mesangial cells,the percentage of cells with G1 phase significantly increased and the percentage of cells with S phase significantly decreased（P0.01）,and its showed dose-dependently.Conclusion：Leflunomide（A771726）could significantly inhibit the proliferation and promote the apoptosis of rat glomerular mesangial cells,and its showed dose-dependently.