钾离子通道阻滞剂4-AP对人舌鳞癌Tca8113细胞增殖的影响

The effects of potassium channel blocker 4-AP on the proliferation of human tongue squamous cell carcinoma Tca8113 cells

目的：检测4-氨基啶（4-AP）对Tca8113细胞株增殖的影响。方法：采用CCK-8法检测不同浓度4-AP作用于Tca8113细胞株12、24、48 h后对细胞的增殖抑制率;流式细胞术（FCM）检测各组细胞周期变化。采用SPSS19.0软件对数据进行统计学分析。结果：随着4-AP浓度的增加和干预时间的延长,Tca8113细胞形态发生改变。在浓度5~100 mmol/L范围内作用于细胞12~48 h,4-AP浓度和时间依赖性地抑制了Tca8113细胞的增殖（P〈0.01）。不同浓度4-AP作用于Tca8113细胞株24 h后,G0/G1期的细胞所占的比例显著升高（P〈0.01）;S期细胞所占的比例显著降低（P〈0.01）;4-AP阻滞Tca8113细胞株于G0/G1期。结论：4-AP可能通过调控细胞周期抑制Tca8113细胞株的增殖对细胞株分布有影响。

Objective： To study the effects of a potassium channel blocker 4-Amino pyridine（ 4-AP） on the proliferation of human tongue squamous cell carcinoma Tca8113 cells. Methods： CCK-8 assay was used to detect the proliferation of Tca8113 cells cultured with 4-AP at the concentration of 1,5,10,20,50 and 100 mmol / L for 12,24 and 48 h respectively,the cell proliferation inhibition rate was calculated,the cell cycle distribution was examined by flow cytometry. Data were analyzed by SPSS19. 0 software. Results：With the increase of 4-AP concentration and culture time,cells showed some morphologic changes. 4-AP at 5- 100 mmol / L dose and time dependently inhibited the proliferation,with 24 h exposure dose dependenty decreased S-phase population and increased G0 / G1 phase population of Tca8113 cells. Conclusion： 4-AP may inhibit Tca8113 cell proliferation by regulation of the cell cycle distribution.