当归水溶性低分子质量部分抗炎作用研究

Study on antiinflammatory effects of water soluble substance with low molecular weight from Angelica sinensis

目的:探讨当归水溶性低分子质量部分抗炎作用的研究。方法:首先取80只SD大鼠随机分为空白对照组、模型组、氢化可的松2 mg·kg-1组和当归水溶性低分子质量部分0.18 g·kg-1、0.09 g·kg-1组。按1 mg·kg-1脂多糖(LPS)腹腔注射制作炎症模型,ELISA测定血浆肿瘤坏死因子(TNF-α)、白细胞介素(IL-6),比较各组给药前后TNF-α和IL-6的差异。然后取40只KM小鼠随机分为4组进行二甲苯致小白鼠耳肿胀试验,比较各组耳肿胀度。结果:与模型组比较,当归水溶性低分子质量部分组TNF-α、IL-6水平明显降低(P<0.05);当归水溶性低分子质量部分可明显抑制二甲苯致小白鼠耳肿胀(P<0.01)。结论:当归水溶性低分子质量部分具有明显的抗炎作用。

OBJECTIVE To study effects of water soluble low molecular weight fractions of Angelica sinensis.METHODSFirst 80 SD rats were randomly divided into the following groups：blank control group,model group,hydrocortisone 2 mg·kg^-1 group,Angelica solution of low molecular weight part 0.18 g·kg^-1 and0.09 g·kg^-1 groups.LPS1 mg·kg^-1 was injected intraperitoneally to produce inflammatory models.ELISA was used to determine TNF-αand IL-6.Then,40 KM mice were randomly divided into 4 groups to carry out xylene induced mouse ear swelling test,and to compare swelling degree between different groups.RESULTS Compared with model group,TNF-αand IL-6 levels in water soluble low molecular weight fractions of Angelica sinensis were significantly decreased（P〈0.05）,and water soluble low molecular weight fractions of Angelica sinensis could inhibit ear swelling of mice induced by xylene（P〈0.01）.CONCLUSION Water soluble low molecular weight fractions of Angelica sinensis have obvious antiinflammatory effects.