中国裸燕麦品种色氨酸含量的荧光检测与评价

Fluorescence Detection and Assessment on Tryptophan Content of Naked Oat Varieties in China

本试验旨在对我国主要裸燕麦栽培品种中色氨酸的含量水平进行评价,并分析其与蛋白质含量的关系。利用木瓜酶对新鲜燕麦粉进行水解,在激发波长288 nm和发射波长352 nm处检测水解产物的荧光强度,根据标准曲线对色氨酸含量进行定量。利用全自动蛋白质分析仪检测籽粒中总氮的含量来定量蛋白质,同时对9个裸燕麦栽培品种在4个不同产区种植的蛋白质和色氨酸含量分别进行检测并作显著性差异和相关性分析。结果表明,中国裸燕麦品种平均的色氨酸含量为2.39 mg/g,大于国外报道的皮燕麦品种平均色氨酸含量1.70 mg/g。品种和地区以及两者互作引起的燕麦色氨酸含量的差异显著,且燕麦色氨酸和蛋白质含量之间具有一定的相关关系,相关系数为0.353。

The experiment aims to assess the tryptophan content of main cultivars of naked oat in China,and analyze the correlation between contents of tryptophan and protein. Oat flour was hydrolysed with papain,the fluorescence intensity of hydrolysate was detected at excitation wavelength of 288 nm and emission wavelength of 352 nm,and finally the tryptophan content was quantitated according to standard curve. The protein was quantitated by using the automatic protein analyzer to test the total nitrogen content of grain,meanwhile the contents of protein and tryptophan were tested respectively for 9 naked oat cultivars growing in 4 different habitats in China,and significant difference and correlation were analyzed. The result showed the average tryptophan content of naked oat varieties in China（ 2. 39 mg /g） was higher than that of hulled oat varieties（ 1. 70 mg /g） reported abroad. There was significant difference in tryptophan content of oat caused by varieties,habitats and interaction,and there was a correlation with the correlation coefficient being 0. 353 between contents of tryptophan and protein. Compared with the hulled oat,Chinese naked oat has an advantage in tryptophan content,which would provide a certain amount of information in oat breeding and processing and utilization.