摘要
探究microRNA-124a(miR-124a)对脊髓损伤(spinal cord injury,SCI)继发性损伤的影响及相关机制.取90只健康成年Wistar大鼠建立SCI模型,随机分为3组,第1组术后仅注射生理盐水,为未治疗组;第2组术后注射骨髓基质细胞源神经干细胞(BMSCs-D-NSCs)治疗,为正常移植组;第3组术后注射携带miR-124a的骨髓基质细胞源神经干细胞(miR-124a-BMSCs-D-NSCs),为转染移植组.另选取60只健康成年Wistar大鼠作为对照组,仅给予假手术处理.采用HE染色观察大鼠的组织形态学变化,采用实时荧光定量PCR技术检测大鼠的miR-124a表达情况,并对损伤灶进行显微测量计算,比较脊髓功能恢复的BBB评分情况及脊髓组织损伤大小的情况.结果显示,SCI后,脊髓组织中miR-124a基因表达显著降低,脊髓神经功能也明显降低,miR-124a-BMSCs-D-NSCs能够有效地改善SCI,减少脊髓损伤组织范围及体积,还能改善中枢神经的功能.
How miR-124 a affects the secondary injury of spinal cord injury（ SCI） and its related mechanism were studied. SCI model was established by gathering 80 healthy adult Wistar rats,which were divided into three groups. The first group,injected with saline only after operation,was the untreated group. The second group,injected with BMSCs-D-NSCs for treatment after operation,was the normal transplantation group. The third group,injected with miR-124a-BMSCs-D-NSCs for treatment after operation,was the transfected transplantation group. Another 60 healthy adult rats were used as the control group,which only accepted sham-operation. Histomorphology change was observed via HE staining,the miR-124 a expression was tested via quantitative real-time PCR,and the lesion site was micro-measured and calculated. The result showed that after SCI,the expression of miR-124 a in spinal cord tissue remarkably decreased,so did the spinal cord nerve function. miR-124a-BMSCs-D-NSCs can,decrease the injured area and size of spinal cord and improve the function of CNS.
出处
《郑州大学学报（理学版）》
CAS
北大核心
2016年第1期73-80,共8页
Journal of Zhengzhou University:Natural Science Edition
