毒结清口服液对Lewis肺癌小鼠血小板活化机制的影响

Effect of Dujieqing Liquor(毒结清口服液) on Mechanism of Platelets Activation in Mice with Lewis Lung Cancer

目的:探讨毒结清口服液对Lewis肺癌小鼠血小板活化标志物表达的影响。方法:建立Lewis肺癌小鼠模型,按分组情况分别干预21 d,观察各组瘤质、抑瘤率及CD62P、CD63表达和v WF水平的变化。结果:毒结清组小鼠体质量显著性高于模型组(P<0.05),毒结清组的抑瘤率为63.2%,化疗组的抑瘤率为38.1%;模型组的CD62P、CD63和v WF水平显著高于正常组(P<0.05);毒结清组的CD62P、CD63表达和v WF水平明显低于模型组和化疗组(P<0.05),显著高于正常组(P<0.05)。结论:毒结清口服液可抑制Lewis肺癌小鼠的肿瘤增殖,保持体质量,可能与其抑制血小板活化机制相关的CD62P、CD63表达和降低v WF水平有关。

Objective： To observe the effects of Dujieqing Liquor on mechanism of platelets activation and metastasis in mice with Lewis lung cancer. Methods： Lewis lung cancer model in mice was established; The tumor inhibiting rate, the inhibiting rate of lung metastasis and change of weight were observed regularly in 21 days after inoculate and the levels of CD62 P, CD63 and v WF were detected in peripheral blood of mice by FCM method. Results： The Dujieqing Liquor group showed higher body weight than the model group（P〈0.05）.The tumor inhibition rate was 63.2% in Dujieqing Liquor group, and 38.1% in DDP chemotherapy group. The model group showed higher level of v WF, CD62 P and CD63 than the normal group（P 0.05）; The Dujieqing Liquor group showed lower level of v WF, CD62 P and CD63 than the model group and DDP chemotherapy group（P〈0.05）, and higher level than nomal group（P〈0.05）. Conclusion： Dujieqing Liquor can inhibit the tumor growth in Lewis lung cancer mice and keep body weight, which may depend on reducing the levels of v WF,CD62 P and CD63.