负载CGRP及SP的明胶缓释微球用于修复家兔骨质疏松模型骨缺损的研究

Sustain-released Gelatin Microspheres Loaded with CGRP/SP for Repairing Bone Defect of Osteoporosis Rabbits

目的:将负载不同浓度的降钙素基因相关肽(CGRP)及P物质(SP)的明胶缓释微球植入骨质疏松兔的股骨缺损中,观察其对骨缺损修复的影响。方法:通过去势方法建立兔骨质松模型,利用乳化交联的方法制备负载不同浓度的CGRP及SP的明胶缓释微球。将24只骨质疏松兔随机分组,于股骨髁部制备骨缺损并分别植入负载10-6 mol/L、10-8 mol/L和10-10 mol/L的SP以及CGRP的明胶微球,3个月后处死观察结果。结果:负载不同浓度的CGRP及SP均能有效地促进成骨,影像学结果示SP在10-6 mol/L时其骨体积分数最高,并且能有效提高骨小梁数量,降低骨小梁间的间距(P<0.05),CGRP在浓度为和10-8 mol/L时效果即达到上述效果;而组织学结果示SP和CGRP均在浓度为10-6 mol/L时其新生骨组织比例最高(P<0.05)。结论:负载不同浓度的CGRP及SP均能有效地促进骨质疏松兔骨缺损的成骨,在所研究的浓度中,高浓度的效果优于低浓度的效果。

Objective：To evaluate the impact of sustain-released gelatin microspheres loaded with CGRP/SP on the osteogenesis of the bone defect in osteoporosis rabbits.Methods：New Zealand white rabbit model was established by ovariectomy.The DEXA was used to detect the rabbits lumbar vertebrae BMD.Sustain-released gelatin microspheres loaded with 10^-6 mol/L,10^-8 mol/L and 10^-10 mol/L of CGRP/SP were prepared via emulsion crosslinking method.Twenty-four rabbit models were randomly assigned to receive sustain-released gelatin microspheres loaded with different concentrations of CGRP/SP into the bone defects.The release in vitro within 1month was measured by ELISA method.The rabbits were killed 3 months after the implanting to evaluate the impact of osteogenesis.Results：Gelatin microspheres loaded with CGRP/SP had better effect than the blank control group.The effect reaches the best when the concentration of SP was 10^-6 mol/L and CGRP was 10^-8 mol/L.And the effect reached the best when the concentration of SP and CGRP was 10^-6 mol/L.Conclusion：Gelatin microspheres loaded with CGRP/SP could improve the new bone formation significantly in contrast to the blank control group.Higher concentration was better.