乳铁蛋白对牵张成骨促进作用的研究

Effects of Lactoferrin on Promoting Bone Regeneration during Distraction Osteogenesis

目的:研究乳铁蛋白(LF)对牵张成骨的作用。方法:将40只新西兰大白兔随机分为对照组(20只)和实验组(20只),建立牵张成骨动物模型。对照组自延迟期第2天开始每日灌胃0.9%氯化钠溶液(1 ml/kg),相同时间内,将LF(85mg/kg)溶解于相同容积的0.9%氯化钠溶液中对实验组进行灌胃。于牵张结束后8周处死所有实验动物,收集标本进行X线、Mirco-CT、组织学和生物力学检测。结果:实验组动物牵张间隙内新骨形成的量优于对照组,并显著改善了新生骨痂的显微结构及生物力学强度。结论:LF能够促进牵张成骨过程中新骨的形成。

Objective：To investigate the effects of lactoferrin（LF）on distraction osteogenesis（DO）.Methods：Forty New Zealand white rabbits were used to establish the DO animal model,and randomly divided into control group（n=20）and experimental group（n=20）.At the beginning of distraction,the rabbits were daily administrated by gavage with LF（85mg/kg）in experimental group,while 0.9% physiological saline（1 mL/kg）in control group.At the 8th week after finishing the distraction,animals were euthanatized,and the samples were collected and preceded to the X-ray,micro-CT,histological,and biomechanical examinations.Results：The radio-density of experimental group was much higher than that of control group.In addition,LF treatment increased the microstructure and mechanical strength of the newly formed bone.Conclusion：LF could promote the bone regeneration during the process of DO.