纳米羟基磷灰石表面阿伦膦酸钠和I型胶原蛋白复合膜对成骨细胞形态和增殖的影响

Effect of Alendronate sAium and Collagen Type I Complex Films of Nano-hydroxyapatite Surface on Osteoblasts Morphology and Proliferation

目的:探讨经化学结合阿伦膦酸钠(alendronate sAium,ALN)和I型胶原蛋白(collagen type I,COL I)修饰的纳米羟基磷灰石(nano-hydroxyapatite,nHA)涂层对成骨细胞在其表面黏附、生长和增殖的影响。方法:建立nHA涂层、nHA/ALN、nHA/COL I、nHA/ALN/COL I和nHA/COL I/ALN复合膜,将成骨细胞接种于复合膜表面,分别培养7d和14d,扫描电镜观察成骨细胞在不同膜层表面的黏附形态的变化,WST-1法对各膜层表面细胞数量进行定量检测。结果:成骨细胞在nHA/ALN/COL I和nHA/COL I/ALN复合膜表面的增殖活性最高(P<0.05),扫描电镜观察成骨细胞在nHA/ALN/COL I和nHA/COL I/ALN复合膜表面生长状态优于其他膜层,在COL I膜直接作用下,成骨细胞伸展更为完全,并有较多伪足突起和微绒毛结构呈分化表型。结论:将ALN和COL I修饰于nHA表面能够协同促进成骨细胞的黏附和增殖。nHA/ALN/COL I复合膜促进成骨细胞的黏附与分化。

Objective：To investigate osteoblast adhesion,growth and proliferation on the alendronate sAium（ALN）and collagen type I（COL I）coated nano-hydroxyapatite（nHA）surface.Methods：nHA layer,nHA/ALN complex film,nHA/COL I complex film,nHA/ALN/COL I complex film,and nHA/COL I/ALN complex film were established.Osteoblasts were cultured on the films for 7days and 14 days.The morphology of osteoblast was observed by SEM and the cell proliferation was determined by WST-1assay,respectively.Results：The results revealed that nHA/ALN/COL I and nHA/COL I/ALN complex films accelerated osteoblasts growth and proliferation rate（P〈0.05）.The osteoblasts showed fully extended morphology combined with numerous pseudopodia and microvilli structure by directly contact to the collagen layer.Conclusion：ALN and COL I exhibited synergistic effect in accelerating adhesion and proliferation of osteoblasts in vitro.The nHA/ALN/COL I complex film benefited osteoblast adhesion and differentiation.