显微镜观察药物敏感性技术快速检测痰标本中结核分枝杆菌的研究

Evaluation microscopic observation drug susceptibility assay in detecting mycobacterium tuberculosis in sputum

目的探讨显微镜观察药物敏感性技术(MODS)在痰液标本中结核分枝杆菌(MTB)的检测效果,以评价其临床应用价值。方法收集150份患者临床痰标本,应用MODS技术、罗氏培养法进行MTB的检测,将两法的结果进行对比分析。结果 MODS技术与罗氏培养法结果符合率为77.8%,MODS技术检测痰标本中MTB的敏感性为93.3%,特异性为70.0%,阳性预测值(PPV)为60.8%,阴性预测值(NPV)为95.4%,正确性为77.8%。结论 MODS技术检测痰标本中MTB具有快速、灵敏、简便等优点,可作为结核病细菌学快速检测的新方法之一。

Objective To study the effect of microscopic observation drug susceptibility assay（MODS）for testing Mycobacterium tuberculosis in sputum,and to evaluate its clinical value.Methods A total of 150 sputum samples were collected from patients with pulmonary tuberculosis,and Mycobacterium tuberculosis were detected by Lowenstein-Jensen（L-J）method and MODS,and compared the results of the two methods.Results The concordance rates of the test results of sputum sample between MODS and L-J was 77.8%.The sensitivity and specificity of MODS were 93.3% and 70.0%,positive predictive value（PPV）was 60.8%,negative predictive value（NPV）was 95.4%,as well as accuracy by MODS was 77.8%.Conclusion MODS assay could be used as a rapid tuberculosis detection method,with rapid,sensitive,simple,and other advantages.