实时荧光定量聚合酶链反应检测食管癌患者血清miR-100表达的意义

Value of real-time fluorescent quantitative polymerase chain reaction in detecting expression of miR-100 in patients with esophageal cancer

目的检测食管癌患者与健康人血清中miR-100的表达量差异,并探讨其作为食管癌分子诊断指标的可能性。方法采用实时荧光定量PCR方法,检测40例食管癌患者（研究组）和50例体检健康者（对照组）血清中miR-100的表达量,并进行统计学分析。结果研究组及对照组血清中miR-100的表达量分别为6.399±3.541、2.625±1.515,研究组明显高于对照组,差异有统计学意义（t=9.07,P〈0.05）。用于食管癌患者诊断的miR-100的ROC曲线下面积为0.832（95%置信区间为0.731-0.934）,当Cut off值为5.285时,miR-100诊断食管癌的灵敏度和特异度分别为65%和95%。结论食管癌患者血清中miR-100表达较健康人高,有望成为该疾病辅助诊断的新分子标志物。

Objective To compare the expression of serum miR-100 in patients with esophageal cancer and healthy person,and explore the value of miR-100 in diagnosis for esophageal cancer.Methods Real-time fluorescent quantitative polymerase chain reaction was used to detecting miR-100 in 40esophageal cancer patients（study group）and 50 healthy person（control group）.ResultsThe expression of miR-100 in the study group and control group were 6.399±3.541,2.625±1.515 respective,the expression in the study group was significant higher than that of the control group（t=9.07,P〈0.05）.The under area of receiver operating characteristic curve of miR-100 in diagnosis for esophageal cancer was 0.832（95% confidence interval was 0.731-0.934）,when the Cut off value was 5.285,the sensitivity and specificity of miR-100 in diagnosis for esophageal cancer were 65% and 95%.Conclusion Serum miR-100 in esophageal cancer patients is higher than that in healthy person,which might be a new molecular markers in diagnosis for esophageal caner.