乳酸杆菌的表面特性及其黏附能力的研究

Surface properties and adhesion ability of Lactobacilus

乳酸杆菌是肠道益生菌,主要通过其表面的黏附因子定殖于肠道而发挥益生作用。为了研究乳酸杆菌表面性质与黏附能力之间的关系,选择五种乳酸杆菌,分别进行自聚集能力、表面疏水性及与致病菌大肠杆菌ATCC25922和枯草芽孢杆菌K的共聚能力测定。同时,利用氯化锂和高碘酸钠分别处理乳酸杆菌后再与致病菌进行共聚作用,研究乳酸杆菌表面参与黏附的活性物质。结果表明,约氏乳酸杆菌F0421和副干酪乳酸杆菌M5-L具有良好的表面性质和黏附特性。乳酸杆菌与枯草芽孢杆菌K的共聚作用较好。同时,氯化锂和高碘酸钠处理前后,乳酸杆菌对致病菌的聚集能力有所下降,表明菌株表面蛋白及多糖参与了黏附过程。

Lactobacillus are the intestinal probiotics and they usually play an important role in intestinal through the ability of adhesion and colonization.In order to realize the relationship between surface properties and adhesion, the autoaggregation ability,copolymerization ability with Escherichia coli ATCC25922 or Bacillus subtilis K and cell surface hydrophobicity of five Lactobacillus strains from different sources were assessed.At the same time, the compounds associated with the adhesion process were investigated by using LiCI-treated and sodium periodate- treated,The results showed that L.paracasei subp.paracasei MS-L and L.johnsonii F0421 showed better surface properties and adhesion ability. When Lactobacillus coaggregated with Bacillus subtilis K had much better copolymerization.Meanwhile, when treated with LiCI or sodium periodate, the autoaggregation and adhesion of Lactobacillus were decreased,indicating that the surface layer protein and polysaccharide play an important role in the process of adhesion.