长双歧杆菌耐氧菌株选育及其高密度发酵条件的研究

Breeding of oxygen- resistant Bifidobacterium longum and its high- density fermentation conditions

通过对长双歧杆菌的紫外诱变,选育出耐氧菌株,命名为L80。以L80为实验菌株,通过单因素实验、响应曲面分析和流加补料培养,确定最优培养基方案。高密度培养优化后的方案是(以质量分数计):大豆蛋白胨4.03%,酵母浸粉2.02%,葡萄糖0.95%,水苏糖1.59%。发酵罐最佳流加方式为:p H6.5,糖浓度保持在6.0~8.0 g/L。最终活菌数达到(1.21±0.03)×10^(10)cfu/m L。

Through the method of ultraviolet mutagenesis to Bifidobacterium Iongum, a strain was screened with resistance ability to oxygen,the strain was named 1.80.Using L80 as the test strain,the optimum culture medium was determined by single factor, response surface and flow and feeding method. High density culture medium optimization formula was （ mass fraction） soy peptone 4.03%, yeast extract powder 2.02%, glucose 0.95% and stachyose 1.59%.The optimal fed-batch cultivation wasthe sugar concentration was kept in 6.0-8.0 g/L, pH6.5, and the final viable bacteria number was reached（1.21 ±0.03） × 10^10 cfu/mL.