不同分子量的羧甲基壳聚糖对糖尿病小鼠血糖的影响

Effect of different molecular weight of CM- chitosan on blood glucose in diabetic mice

目的:研究不同分子量的水溶性羧甲基壳聚糖对糖尿病小鼠的降血糖作用。方法:通过腹腔注射链脲佐菌素160 mg/kg建立小鼠糖尿病模型,并将造模成功的小鼠随机分为模型组、阳性对照组、实验组(高分子量羧甲基壳聚糖组、中分子量羧甲基壳聚糖组、低分子量羧甲基壳聚糖组),设置空白对照组,每组12只。实验组小鼠每日按990 mg/kg·b·w分别灌胃不同分子量羧甲基壳聚糖,模型组和空白对照组按体质量灌胃相同体积的蒸馏水,阳性对照组按20 mg/kg·b·w灌胃拜唐苹阿卡波糖,连续灌胃28 d,于末次灌胃后测定小鼠空腹血糖值、糖耐量。结果:不同分子量的羧甲基壳聚糖对高血糖模型小鼠体重没有显著影响(p>0.05);与模型组比较,低分子量组能极显著的降低造模小鼠的血糖浓度(p<0.01),中分子量组能显著的降低造模小鼠的血糖浓度(p<0.05),而高分子量组未见显著效果;并且三者均可改善糖尿病小鼠的糖耐量。综上所述,低分子量羧甲基壳聚糖降血糖效果最好,中分子量羧甲基壳聚糖效果次之,高分子量羧甲基壳聚糖效果最弱。

Objective ：To study the effect of CM-chitosan with different molecular weight on blood glucose in diabetic mice.Methods：The diabetic model was established by intraperitoneal injection of 160 mg/kg STZ（Streptozocin）, and the mice were randomly divided into model group, positive control group, experimental group（ high molecular weight CM-chitosan group, middle molecular weight CM-chitosan group, low molecular weight CM-chitosan group） ,and blank control group, with 12 rats in each group. Experimental group were daily given experimental drugs according to 990 mg/kg.b.w, model group and blank control group were daily given the same volume of distilled water,the positive control group were daily given acarbose tablets according to 20 mg/kg.b.w,totally for 28 days.After the last time, the fasting blood glucose and the glucose tolerance were measured. Results： The different molecular weight of CM- chitosan had no significant effect on the body weight of mice （ p 〉 0.05 ）. Compared with the model group, the low molecular weight group could significantly reduce the blood glucose concentration（p 〈 0.01 ）, and the molecular weight can reduce the blood glucose concentration （p 〈 0.05）, but no significant effect of high molecular weight group, and the glucose tolerance of diabetic mice were improved in all the experimental group. In summary, the effect of low molecular weight CM- chitosan was the best, the middle molecular weiaht CM-chitosan was the second,the hiClh molecular weight CM-chitosan was the last.