鹅掌楸树叶和树皮提取物的抑菌活性研究

Anti-bacterial effects of extracts from the Liriodendron L. barks and leaves

林源生物活性物质的研究和利用是森林资源利用的重要方面。为研究重要的药源植物鹅掌楸属植物树皮及树叶提取物的抑菌活性,用琼脂稀释法检测鹅掌楸属不同种间和种内树皮及叶片提取物对大肠杆菌、绿脓杆菌、金黄色葡萄球菌和粪链球菌的抑菌反应。结果表明:鹅掌楸属3个树种的树皮和树叶提取物对大肠杆菌、绿脓杆菌、金黄色葡萄球菌和粪链球菌有明显的抑菌效果;树皮提取物对大肠杆菌、绿脓杆菌、金黄色葡萄球菌及粪链球菌的最低抑菌质量浓度(MIC)分别为5.12、0.64、0.64和0.64 mg/mL;树叶提取物的MIC分别为40.96、2.56、2.56和2.56 mg/mL。鹅掌楸属树种不同部位的提取物抑菌效果有明显差别,树皮提取物抑菌效果强于树叶提取物,但不同种间提取物的抑菌效果差异不显著。

Forest plant is one of the important medicine resources. Liriodendron L. is not only an important timber pro- ductive genus with superior wood quality, but also an important traditional Chinese medicine. Here, we report the results of anti-bacterial activities of extracts from Liriodendron L. barks and leaves. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus faecalis were test strains, and the anti-microbial activities of the extracts were determined by agar dilution methods. The results showed that the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus faecalis on agar were obviously inhibited by adding extracts from Liriodendron L. barks and leaves. The MIC of the barks extracts were 5.12, 0.64, 0.64 and 0.64 mg/mL, respectively, and the MIC of the leave extracts were 40.96, 2.56, 2.56 and 2.56 mg/mL, respectively. The anti-microbial activities of the extracts from the barks were more effective than that of the leaves extracts. However, the anti-microbial activities among species within the Liriodendron L. were not significant statistically.