日本晴/R1126水稻重组自交系群体粒形性状QTL定位

Mapping of QTL Associated with Grain Shape Traits in Nipponbare/R1126 Recombinant Inbred Lines Population of Rice

为阐明大穗型籼稻品系R1126稻谷籽粒性状的遗传机制,以其与粳稻日本晴构建的重组自交系F10为材料,通过连续2年田间种植并测定各株系的籽粒粒长、粒宽和粒厚等表型性状,结合利用SSR和SFP等分子标记构建的遗传图谱,对控制该群体的稻谷粒形性状进行了QTL分析。2年试验共检测到10个控制粒长、粒宽和粒厚性状的QTL,其中q GL3-1、q GL3-2和q GL9这3个粒长QTL,以及q GW2和q GW5这2个粒宽QTL在2年试验中能被重复检测;而粒厚性状在2年试验中检测到5个QTL,但均只在1年试验中出现。根据连锁的分子标记信息,q GL3-2、q GW2和q GW5可能分别与已报道的主要粒形基因GS3、GW2和GW5等位;而q GL3-1和q GL9可能为新的粒长QTL,且在2年试验中具有很好的重演性和稳定性,两者的加性效应均能使粒长增加0.2 mm以上,对于改善稻谷外观品质性状具有较好的潜在应用价值。

In order to clarify the genetic mechanism of grain shape traits for large panicle indica rice line,R1126,a set of recombinant inbred lines,which contain 185 lines,was developed by using R1126 crossed to the japonica rice line,Nipponbare. By measuring the grain shape traits include grain length（ GL）,grain width（ GW） and grain thickness（ GT） in two years field experiment,combining with the genetic map constructed with SSR and SFP molecular markers,QTL associated with grain shape traits were analyzed. Total ten QTL associated with GL,GW and GT,were detected in two years experiment. Among them,three QTL related to GL including q GL3-1,q GL3-2 and q GL9,and two QTL related to GW including q GW5 and q GW2,were be detected repeatedly in two years,respectively. However,no one QTL related to GT could be detected repeatedly. By comparing the molecular marker information,q GL3-2,q GW2 and q GW5 might be allele with the reported genes of GS3,GW2 and GW5,respectively. The two QTL associated with grain length,q GL3-1 and q GL9,which were expressed stably and repeated in two years experiment,exhibiting the additive effect of increasing the grain length of 0. 2 mm,could be used for improving rice appearance quality.