摘要
研究了两步酶法催化制备1-十八烷氧基-2-DHA-3-油酸烷氧基甘油二酯工艺。首先利用脂肪酶Lipozyme 435催化1-十八烷氧基甘油和DHA乙酯合成1-十八烷氧基-2,3-二DHA烷氧基甘油二酯(DEAG1),然后利用脂肪酶Lipozyme RM IM催化油酸乙酯和DEAG1进行酯交换反应制备1-十八烷氧基-2-DHA-3-油酸烷氧基甘油二酯。通过单因素实验研究了反应温度、反应时间、底物摩尔比和加酶量对酯交换反应的影响,得到了最佳工艺条件。结果表明:在反应时间48 h、DHA乙酯与1-十八烷氧基甘油摩尔比2∶1、加酶量为底物总质量的10%、反应温度50℃条件下,合成的DEAG1含量最高,为75.41%;在反应时间12 h、油酸乙酯与DEAG1摩尔比3∶1、加酶量为底物总质量的7%、反应温度60℃条件下,Sn-3位油酸结合率为30.23%,烷氧基甘油二酯总含量为80.49%。
Two-step lipase-catalyzed preparation of 1-O-octadecyl-2-DHA-3-oleic acid alkoxy diacylglycerol was studied. First,1-O-octadecyl-2,3-di DHA alkoxy diacylglycerol（ DEAG1） was synthesized by 1-O-octadecyl glycerol and DHA ethyl ester catalyzed by Lipozyme435. Then,1-O-octadecyl-2-DHA-3-oleic acid alkoxy diacylglycerol was prepared by DEAG1 and ethyl oleate catalyzed by Lipozyme RM IM. The effects of reaction temperature,reaction time,molar ratio of substrate and dosage of enzyme on the transesterification were studied by single factor experiment,and the optimal process conditions were obtained. The results showed that under the conditions of reaction time 48 h,molar ratio of DHA ethyl ester to 1-O-octadecyl glycerol 2 ∶ 1,dosage of enzyme 10% of the total mass of substrate and reaction temperature 50 ℃,the content of DEAG1 synthesized was the highest,up to 75. 41%. Under the conditions of reaction time 12 h,molar ratio of ethyl oleate to DEAG1 3 ∶ 1,dosage of enzyme 7% of the total mass of substrate and reaction temperature60 ℃,the combination rate of oleic acid in Sn-3position was 30. 23% and the total content of alkoxy diacylglycerol was 80. 49%.
出处
《中国油脂》
CAS
CSCD
北大核心
2016年第3期21-25,共5页
China Oils and Fats
基金
国家自然科学基金项目(31201329)
长江学者创新团队发展计划资助(IRT1188)
