一株耐氧耐酸碱高效异养硝化菌的筛选及其硝化特性研究

Research on Screening and Nitrification Characteristics of a Efficiently Heterotrophic Nitrifier Resistanting Oxygen,Acid and Alkali

通过专一性选择培养基,从活性污泥中筛选到一株高效异养硝化菌,并对菌株进行了鉴定及硝化特性研究。结果表明,筛选到的异养硝化菌株为芽胞杆菌属（Bacillus sp.）,命名为GL2。碳源、有机氮源、C/N、溶解氧及初始pH值均对菌株硝化特性有较大影响,在丁二酸钠为碳源、硫酸铵为氮源、C/N15、DO 3.8～8.5mg/L及初始pH值为6～10、氨氮负荷为420mg/L条件下,氨氮去除率达99.7%,显示出该菌株高效硝化能力。菌株兼具好氧反硝化特性,可实现单级反应器的同步硝化反硝化,具有潜在的工程废水处理应用价值。

A heterotrophic nitrifier is isolated from the activated sludge of landfill leaehate through specific select medi- um. It is identified as Bacillus sp. named as GL2. Its nitrification function is studied to show that the factors including carbon source, C/N, pH, DO and organic nitrogen are important for nitrification of strain GL2. The optimized condition for nitrification is as follows： sodium suceinate as the carbon resource, C/N of 15, pH of 6-10, DO of 3.8-8.5 mg/Land the initial ammonia nitrogen of 420 mg/L. Combining these conditions, the removal rate of ammonia nitrogen reaches 99.7 %. The strain has the capability to achieve aerobic denitrification. These results show that strain GL2 with highly effective heterotrophie nitrification could achieve simultaneous nitrification and denitrification. Hence, the strain GL2 could have high potential in practical wastewater treatment.