绿原酸抑制金黄色葡萄球菌机理研究

Study on the Mechanism of Chlorogenic Acid Inhibiting Staphylococcus aureus

为探索绿原酸(CGA)抑制金黄色葡萄球菌增殖的机制,通过检测β-半乳糖苷酶的变化反映细菌细胞膜通透性的变化,凝固酶的活性反映细菌致病力;用Bradford法、DNS法和DNP法分别检测可溶性蛋白质量浓度、还原糖利用能力和丙酮酸质量浓度的变化反映细菌代谢的改变.结果显示CGA处理金黄色葡萄球菌后菌液中β-半乳糖苷酶出现泄漏,且漏出量与CGA质量浓度呈量效关系,无血浆凝固现象,菌液中还原糖质量浓度急剧升高,可溶性蛋白质量浓度降低量超过50%,丙酮酸质量浓度升高5.34%,与对照组相比差异极具统计学意义(p<0.01),表明CGA可能通过改变金黄色葡萄球菌细胞膜的通透性,阻碍物质和能量的代谢及细菌蛋白合成而达到抑菌作用.

To explore the mechanism of chlorogenic acid（CGA）inhibiting the proliferation of Staphylococcus aureus,the change inβ-galactosidase enzyme was monitored to reflect the change in membrane permeability of S.aureus,the coagulase activity was detected to reflect bacterial virulence,and the changes in the content of soluble proteins,reducing sugar utilization and pyruvate content were tested with Bradford,DNS and DNP methods,respectively,to reflect the changes in bacterial metabolism.After the treatment of S.aureus with CGA,β-galactosidase enzyme leakage was noticed in the bacterial liquid with a dose-response relationship.No plasma coagulation was detected,the content of reducing sugars increased sharply,soluble protein content was reduced by more than 50%,and pyruvate content increased by 5.34%in the bacterial liquid.The differences compared with the control group were highly significant statistically.The study concluded that the CGA could inhibit the proliferation of S.aureus and that its main antibacterial mechanism might be to change the membrane permeability of this bacterium,inhibit material and energy metabolism and retard protein synthesis.