纳米粒子三明治型传感器的组装及其在BRCA-1检测中的应用

Nanoparticles-fabricated Sandwich Biosensor for Single-mismatched BRCA-1 Detection

采用纳米磁、DNA、纳米金组装三明治型生物传感器。其中,纳米磁粒子(PMPS)用于捕获并分离目标DNA,而被基因探针序列修饰过的纳米金(Au-NPs)则扮演识别序列及产生信号的角色,从而使目标DNA序列量转化为光信号,继而通过紫外分光光度计进行定量分析。这种基于纳米金修饰的DNA探针的新型基因诊断技术,可以用来定量检测分析目标DNA。结果显示,这是一种非常简单经济且实用的检测单个突变基因序列的新方法。

In this study,a sandwich biosensor consisting of PMP (para -magnetic particles)/DNA /Au -NPs (gold nanoparticles)was fabricated.PMPs captured and separated target DNA while Au -NPs modified with oligonucleotide detection sequences played a role in recognition and signal production.There-fore,quantitative information concerning the target analyte was translated into a colorimetric signal,which could be easily and quantitatively measured by low -cost UV -vis spectrophotometric analysis.The results indicated that it was a very simple and economic strategy for detection of single -mismatched DNA strands.