虎杖免煎颗粒与氟康唑联合抗白色念珠菌效果

The in vitro anti-Candida albicans effect of Polygonum granules combined with fluconazole

目的研究虎杖免煎颗粒与氟康唑的体外联合抗白色念珠菌作用。方法借助临床分离的白色念珠菌13株,通过微量液基稀释法测定虎杖免煎颗粒的抗真菌作用及其与氟康唑的联用时的抗真菌作用。结果虎杖免煎颗粒对氟康唑敏感白色念珠菌的最小抑菌浓度(MIC)平均值为74.67 mg/ml,对耐药白色念珠菌的MIC平均值为237.71 mg/ml;虎杖免煎颗粒与氟康唑联合应用对敏感白色念珠菌表现为相加或无关作用,对耐药白色念珠局表现为协同作用。虎杖免煎颗粒与氟康唑联合应用对敏感白色念珠菌的分数抑菌浓度指数(FICI)平均值为0.81,对耐药白色念珠菌的FICI平均值为0.35。结论虎杖免煎颗粒本身具有一定的抗白色念珠菌作用,与氟康唑联合应用对敏感菌表现为相加或无关作用,对耐药菌表现为协同作用。

Objective To investigate the in vitro antifungal effect of Polygonum granules and fluconazole against Candida albicans. Methods A microdilution method was used to determine the minimum inhibitory concentration（MIC） of Polygonum granules against 13 clinical Candida albicans strains. The combined effect of Polygonum granules and fluconazole was assessed by the fractional inhibitory concentration index（FICI） with checkerboard microdilution method.Results The average MIC was 74.67 mg / ml of Polygonum granules alone against susceptible strains, and that was237.71 mg / ml against resistant ones. The average FICI was 0.81 against susceptible strains, and that was 0.35 against resistant ones. Conclusion Polygonum granules had certain antifungal activity. It acts additive or no interaction with fluconazole against susceptible Candida albicans, and synergistically against resistant strains.