蜂胶对烟曲霉抑菌机制的体外研究

Propolis on Aspergillus fumigatus antifungal mechanisms in vitro

探讨蜂胶对烟曲霉的抗茵作用及其作用机制,为蜂胶临床治疗由烟曲霉引起的疾病提供参考依据。本文采用不同浓度(10、20、30、40、50、100、150、200 mg/m1)的蜂胶乙醇提取物(EEP)处理烟曲霉孢子,结果发现,其存在剂量依赖性,接着琼脂稀释法测定蜂胶的最小抑茵浓度(MIC),将烟曲霉与1/2MIC_(50)EEP孵育3d后发现,蜂胶组烟曲霉菌丝的重量和呼吸速率显著低于对照组,且蜂胶组孢子的超微结构严重受损。通过半定量PCR分析磷脂腺肌醇信号通路相关的表达情况,发现蜂胶组pKC基因与对照组相比呈现下调趋势。这些结果暗示,蜂胶能够抑制烟曲霉的生长,具有治疗由烟曲霉引起的疾病的潜力,是一种有前途的生物活性化合物。

To investigate the effects of propolis on A. fumigatus , so as to provide guidance for clinical treatment of the A. fumigatus infection. Using different concentrations （10, 20, 30, 40, 50, 100, 150, 200 mg/ml） of ethanolic extract of propolis（EEP） to deal with A. fumigatus spores found that a dose-dependent relationship between propolis and bacte- riostatic ring size. Next, the minimum inhibitory concentrations （MIC） of propolis were determined by agar dilution method, A. fumigatus mycelium weights and spores respiratory rate were significantly inhibited when exposure to 1/ 2MIC50 of propolis. Unltrastructure of spore was seriously damaged with propolis incubation tor 3d, which was further confirmed by soluble protein loss of A. fumigatus mycelia treated with propolis. Seni-quantitative PCR found Phosphatidylinositol signal pathway related PLC gene expression down-regulated. These resuhs suggest propolis can inhibit the growth of A. fumigates.and have a promising potential to treat pneumonia caused by A. fumigates.