外源性巨噬细胞对小鼠细菌性腹膜炎的治疗作用

Therapeutic effects of exogenous macrophages in mice with bacterial peritonitis

目的:研究腹腔注射外源性巨噬细胞对细菌性腹膜炎小鼠转归的影响,探讨巨噬细胞对细菌性腹膜炎小鼠的治疗作用,并与抗生素的治疗作用进行比较.方法:预实验确定了大肠杆菌浓度的半数致死量、激活的U937细胞的最大安全浓度及大肠杆菌敏感的庆大霉素最大安全剂量.60只小鼠被随机分为:A组PBS液对照组,B组庆大霉素组,C组激活U937细胞组.观察每组小鼠的体质量变化、一般情况及死亡情况.结果:激活U937细胞或庆大霉素注射的小鼠体质量明显大于PBS液的对照组(P<0.05),一般情况比对照组好(P<0.05),死亡率明显降低;此外激活U937细胞组小鼠体质量明显大于庆大霉素组(P<0.05),一般情况比庆大霉素组好(P<0.05),注射细菌和激活U937细胞的小鼠无死亡,而注射细菌和庆大霉素的小鼠死亡率为20.0%(P<0.01).结论:腹腔注射外源性巨噬细胞对小鼠细菌性腹膜炎有治疗作用,可能优于腹腔注射庆大霉素的治疗作用.

AIM：To determine the therapeutic effects of exogenous macrophages（U937） in mice with bacterial peritonitis.METHODS：Sixty mice were randomized into three groups.Group A received injections of E.coli suspension and PBS.Group B received injections of E.coli suspension and gentamycin.Group C received injections of E.coli suspension and activated U937 cells.All dosages,included median lethal dose（LD50）of E.coli and maximum safe doses of activated U937 cells and gentamycin,were determined by pre-tests.RESULTS：The weight and general condition of mice in groups B and C were significantly better than those of group A（P〈0.05）.The fatality rate in groups B and C was lower than that of group A.Compared with group C,general condition and weight of mice in group B were better.The fatality rate in group B was 0,while that of group C was 20%（P〈0.01）.CONCLUSION：As a treatment for bacterial peritonitis,exogenous macrophages（U937） are better than gentamycin.