摘要
目的探讨核因子-κB(NF-κB)及诱导型一氧化氮合酶(iNOS)、一氧化氮(NO)对氟尿嘧啶(FU)诱导的糖代谢异常的影响。方法 Wistar大鼠40只,随机分为两组,各20只。实验组腹腔注射FU 20mg/(kg·d)连续5d,对照组腹腔注射等量的9g/L氯化钠溶液连续5d,于第2、7天时行口服葡萄糖耐量试验(OGTT),检测血糖及胰岛素水平。然后处死大鼠,每次10只,取胰腺组织,检测iNOS、NO含量及NF-κB表达情况。结果实验组大鼠第2、7天OGTT试验后各时间血糖水平均明显高于对照组(t=5.742~12.993,P〈0.05)。第2、7天时实验组30、60min时胰岛素值低于对照组,120 min时胰岛素值高于对照组,差异均有显著性(t=2.107~8.263,P〈0.05)。实验组第7天时胰岛素分泌高峰延迟至120min(F=34.623,P〈0.05)。与对照组比较,实验组第2、7天NF-κB、iNOS、NO值均增高,差异有统计学意义(t′=8.43~35.00,P〈0.05)。相关分析显示,实验组第2、7天NF-κB与iNOS、空腹血糖值之间存在正相关(r=0.861 1~0.923 4,P〈0.05)。结论 FU引起的糖代谢异常可能与NF-κB引起的氧化应激有关。
Objective To explore the impact of NF-κB,iNOS and NO on dysglycaemia induced by fluorouracil(FU).Methods Forty Wistar rats were evenly randomized to two groups.The rats in experimental group were intra-peritoneal injection of FU 20mg/(kg·d)for 5days,and those in the control group were given intra-peritoneal injection of normal saline with a dosage same as that used in the experimental group.Oral glucose tolerance test(OGTT)was done on days 2and 7after FU administration,blood glucose and plasma insulin were then detected and the rats were sacrificed,with 10 rats each time.Pancreases were removed for detection of iNOS and NO levels and expression of NF-κB. Results Compared with rats in the control group:blood glucose of rats in the experimental group at each time point of OGTT test was higher.On days 2and 7,the level of insuline at 30 and 60min was lower and at 120 min,it was higher,the differences were significant(t=2.107-8.263,P〈0.05).On day 7,the peak time of insuline secretion delayed to 120min(F=34.623,P〈0.05).On days 2and 7,the levels NF-κB,iNOS and NO were all elevated(t′=8.43-35.00,P〈0.05).A correlation analysis showed that,on days 2and 7,the level of NF-κB in the experimental group was positively correlated with iNOS and fasting blood sugar(r=0.861 1-0.923 4,P〈0.05). Conclusion The Dysglycaemia caused by fluorouracil is probably associated with oxidative stress due to NF-κB.
出处
《齐鲁医学杂志》
2016年第1期10-12,共3页
Medical Journal of Qilu