miR-30a-5p在肾癌中的表达分析及临床意义研究

Research on the expression of miR-30a-5p and its clinical significance in renal tumor

目的:本研究通过检测32对肾细胞癌组织标本及相应癌旁正常组织标本中miR-30a-5p的表达水平,然后分析miR-30a-5p的表达量与患者临床特征之间的关系,为更深一步研究miR-30a-5p在肾细胞癌的发病机制的作用和功能的研究打下基础。方法:收集32对经手术切除的肾细胞癌及相应的癌旁组织标本,提取标本的总RNA,经反转录获得cDNA,并通过实时荧光定量PCR(qRT-PCR)方法检测miR-30a-5p的表达水平,利用统计学方法分析其表达量与患者临床特征之间有无相关性。结果:32对组织标本中有27例(84.38%)肾细胞癌组织的miR-30a-5p表达水平下降(P<0.01),且统计学分析表明其下降和患者的年龄、性别、组织学分型及临床分期等临床特征无明显相关性(P>0.05)。结论:miR-30a-5p在肾癌组织中表达明显下调,提示其可能与肾癌的发生发展有一定的相关性。

Objective：To examine the expression levels of miR-30a-5p in 32 RCC tissues and paired normal tissues,and then explore the relationship between miR-30a-5p expression and clinical characteristics of patients for further study of the function of miR-30a-5p in RCC.Method：Total RNA that extracted from 32 paired tissues resected from nephrectomy was used to get cDNA by performing reverse transcription.Quantitative real-time PCR（qRT-PCR）was used to examine the expression level and statistic analysis was used to explore the relationship between clinical characteristic and expression of miR-30a-5p.Result：Expression of miR-30a-5p down-regulated in 27cases（84.38%）（P 0.01）.No relationships could be found between the expression of miR-30a-5p and patients＇ age,gender,histological classification or clinical stage（P 0.05）.Conclusion：Expression of miR-30a-5p downregulated in RCC tissues,which may demonstate the correlation between its expression and the initiation and development of RCC.