富硒嗜酸乳杆菌防治小鼠急性实验性结肠炎及可能机制

The effect and possible mechanism of selenium-enriched Lactobacillus acidophilus on colitis of mice

目的探讨富硒嗜酸乳杆菌防治葡聚糖硫酸钠(DSS)诱导的小鼠急性结肠炎的疗效及可能机制。方法小鼠大肠组织进行病理学损伤评分,应用原子荧光光谱法测定大肠组织中硒含量,Real-time PCR方法检测大肠组织中IFN-γ、IL-17A及IL-10m RNA水平,ELISA方法检测大肠固有层淋巴细胞培养液中IFN-γ、IL-17A及IL-10表达水平。结果与模型组、亚硒酸钠组、嗜酸乳杆菌组相比,富硒嗜酸乳杆菌组小鼠病理学损伤评分显著降低(<0.05);与亚硒酸钠组、嗜酸乳杆菌组相比,富硒嗜酸乳杆菌组小鼠大肠组织硒含量显著增高(<0.05);与模型组、亚硒酸钠组、嗜酸乳杆菌组相比,富硒嗜酸乳杆菌组小鼠IFN-γ、IL-17A表达水平显著降低(<0.05),IL-10的表达水平显著增高(<0.05)。结论富硒嗜酸乳杆菌防治DSS诱导的小鼠急性结肠炎,疗效显著优于亚硒酸钠以及嗜酸乳杆菌,其机制可能与降低IFN-γ、IL-17A,升高IL-10水平有关。

Objective To investigate the effect and possible mechanism of selenium-enriched Lactobacillus acidophilus（SELA）on colitis of mice.Methods The content of selenium in colon tissue was detected by atomic fluorescence spectrometry,the expressions and contents of IFN-γ,IL-17 A and IL-10 mRNA and protein were detected by Real-time PCR and ELISA respectively.Results The histological injury scores were significantly lower in SELA group than in model group or sodium selenite or lactobacillus acidophilus（p〈0.05）.Selenium content in colon tissue was significantly higher in SELA group than in sodium selenite or lactobacillus acidophilus（p〈0.05）.The expression levels of IFN-γand IL-17 A decreased significantly（p〈0.05）,but increased for the expression of IL-10 in SELA mice than in DSS colitis or sodium selenite or lactobacillus acidophilus group.Conclusion The effective therapeutic effect of SELA on DSS-induced acute colitis might be related to the regulatory effect of SELA on IFN-γ,IL-17 A and IL-10.