索拉菲尼对人神经胶质瘤细胞增殖和凋亡的影响

The effect of Sorafenib on the proliferation and apoptosis of human glioblastomas LN229 cells

目的探究索拉菲尼对人脑神经胶质瘤LN229细胞增殖和凋亡的影响,为临床应用提供一定的理论基础。方法体外培养LN229细胞,用CCK8法检测LN229细胞增殖、TUNEL法检测LN229细胞凋亡,Western blot技术检测Cyclin D1、caspase-3、p-ERK1/2、ERK1/2表达。结果索拉菲尼可以明显降低人脑神经胶质瘤LN229细胞活力,降低Cyclin D1和pro-caspase-3表达水平,提高TUNEL阳性率,抑制ERK1/2磷酸化。结论索拉菲尼抑制LN229细胞增殖可能与调节MAPK信号通路相关。

Objective To study the effect and possible mechanism of Sorafenib on the proliferation and apoptosis of human glioblastomas LN229 cells.Methods The proliferation and apoptosis of LN229 cells were detected by CCK8 kit and TUNEL staining respectively,the expressions of Cyclin D1,caspase-3,p-ERK1/2 and ERK1/2 in LN229 cells were determined by Western blot.Results Sorafenib reduced significantly LN229 cells viability,decreased the expression levels of Cyclin D1 and pro-caspase-3,increased the apoptotic rates of LN229 cells and inhibited the activation of MAPK signal pathway.Conclusion The inhibition of Sorafenib on the proliferation of LN229 cells might be related to inhibition of MAPK pathway phosphorylation.