摘要
目的研究重组人生长停滞特异性蛋白6(Gas6)对大鼠肾上腺嗜铬细胞瘤PC12细胞氧糖剥夺/复糖复氧是否有保护作用及可能机制。方法体外培养PC12细胞,随机分为3组:正常对照组(Control组)、氧糖剥夺/复糖复氧模型组(OGD/R组)、Gas6治疗组(Gas6组)。应用MTT法测定细胞活力,比色法测定乳酸脱氢酶(LDH)释放量,分光光度法检测半胱氨酸蛋白酶(Caspase-3)活性变化,流式细胞仪检测细胞凋亡率。结果OGD/R组与Control组比较细胞活力降低,LDH释放量、Caspase-3活性和凋亡率增加(<0.01)。而Gas6组与OGD/R组比较,细胞活力增加,LDH释放量、Caspase-3活性和凋亡率均减低(<0.01)。结论 Gas 6对PC12细胞氧糖剥夺/复糖复氧的保护作用可能与抑制Caspase-3活化抗细胞凋亡相关。
Objective To observe the protective effect of growth arrest-specific protein 6（Gas6）on PC12 cells with oxygen-glucose deprivation/regain and its possible mechanism.Methods Rat adrenal pheochromocytoma PC12 cells were cultured and randomly divided into 3 groups：normal control group,oxygen-glucose deprivation/regain model group（OGD/R group）,Gas6 group.The cells activity was measured by MTT method,lactate dehydrogenase（LDH）release was measured by colorimetric method,cysteine protease caspase-3 activity was detected by spectrometric method,cell apoptosis rate was measured by flow cytometry.Results In OGD/R group,cell viability was decreased,the release of LDH,caspase-3 activity and apoptosis rate were all increased（p〈0.01）,compared with control group;while in Gas 6 group,the cell viability was increased,the release of LDH,caspase-3 activity and apoptosis rate were all significantly decreased,compared with OGD/R group（p〈0.01）.Conclusion The protective effects of Gas 6 on oxygen-glucose deprivation/regain PC12 cells might be related to the inhibition of caspase-3 activity.
出处
《解剖科学进展》
2016年第2期183-185,189,共4页
Progress of Anatomical Sciences
基金
辽宁省科技厅博士启动基金(20131114)
辽宁省教育厅科学技术研究一般项目(L2015535)
沈阳医学院科技基金项目(20133053)
沈阳医学院大学生科研立项(20141035)
