2014年北京市房山区沙门菌血清学鉴定及耐药性分析

Serological identification and drug resistance analysis of Salmonella infection in Fangshan District in 2014

目的分析2014年北京市房山区感染性腹泻患者中沙门菌的血清型及耐药情况,为本地沙门菌感染临床用药提供依据。方法收集房山区2家哨点医院感染性腹泻患者的肛拭子培养,进行沙门菌的生化鉴定和血清分型,用微量肉汤稀释法进行药物敏感性试验。结果共分离出19株沙门菌,检出9个血清型,其中前3位的为猪伤寒沙门菌、鼠伤寒沙门菌和布利丹沙门菌,构成比均为21.05%。药物敏感试验显示19株沙门菌对头孢西丁、头孢噻肟和庆大霉素敏感率较高,分别为100.00%、94.74%和89.47%。对萘啶酸和四环素耐药率较高,分别为63.16%和42.11%。结论北京市房山区2014年沙门菌优势血清型为猪伤寒沙门菌、鼠伤寒沙门菌和布利丹沙门菌,对头孢西丁、头孢噻肟和庆大霉素敏感率较高,临床治疗时可合理使用抗生素。

Objective To analyze the serotype and drug resistance of Salmonella in patients with infectious diarrhea in Fangshan District in 2014,so as to provide reliable evidence for clinical drug- using in Salmonella infection. Methods Anal swab were collected form sufferers with infection diarrhea via two sentinel hospitals in Fangshan district for culture,biochemical testing and serotyping. Micro broth dilution method was used for drug sensitivity test. Results 19 strains of Salmonella were isolated. The strains represented 9 serotypes. Of the strains,S. typhi- suis,S. typhimurium and S. blegdam were all 21. 05% to be the top 3. Susceptibility results for the Salmone strains indicated that susceptibility to cefoxitin was 100. 00%,susceptibility to cefotaxime was 94. 74%,susceptibility to gentamicin was 89. 47%. The resistance rates to tetracycline and tetracycline were higher,accounting for 63. 16%,42. 11%,respectively. Conclusion The predominant pathogens were S. typhi- suis,S. typhimurium and S. blegdam of Salmonella in Fangshan District,Beijing,in 2014,which are highly susceptible to cefoxitin,cefotaxime and gentamicin. So clinical use of antibiotics should be conducted based on the drug sensitive test.