CHO M期同步细胞的研究

STUDIES ON THE CHO M-PHASE SYNCHRONIZED CELLS

引言 1953年,Howard和Pelc以蚕豆根端细胞为材料,用同位素示踪方法摻入32P到蚕豆根细胞DNA中,发现只有在DNA合成时才有32P的掺入,而在DNA合成后到有丝分裂期之间有一间隙（gap）存在,此时32P不能掺入,因此,首先提出细胞周期的概念。1959年Oastler和Sherman利用3H-Td标记小鼠肠腺窝上皮细胞的有丝分裂象的方法测定细胞周期各时相的时间,为细胞动力学的研究提供了重要手段。

1. By means of the method of mechanical shaking in several steps of collection, a lot of M-phase synchronized cells were obtained. 2. The mitotic indices of the cells were found to be greater than 90%. 3. Synchronized cells keep their dividing capacity in an ice-water bath for 4 hrs.